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PReS-FINAL-2020: Cell type specific transcriptome analysis in patients with enthesitis related arthritis category of juvenile idiopathic arthritis (JIA-ERA)
© Aggarwal et al.; licensee BioMed Central Ltd. 2013
Published: 5 December 2013
Enthesitis Related Arthritis Category of Juvenile Idiopathic Arthritis (JIA-ERA) is the most common category of JIA seen in Asian Indians. Transcriptome analysis is a useful tool to analyse pathways involved in disease pathogenesis. Peripheral blood mononuclear cells (PBMC) and SFMC analysis showed involvement of innate immune cells in JIA-ERA. However PBMC/SFMC have variable number of different cells and that can affect interpretation. No data is available on cell type specific transcriptome analysis of blood and synovial fluid in children with JIA-ERA.
To study the cell type specific transcriptome analysis of blood and synovial fluid in children with JIA-ERA.
Six samples each of peripheral blood and synovial fluid were collected from patients with ERA-JIA. Blood from 6 healthy controls was also collected. Mononuclear cells were separated by density gradient centrifugation. B cells, T cells and monocytes were separated using MACS columns and purity assessed by flow cytometry. After RNA extraction and checking the quality of RNA (RIN > 8) microarray was done using Illumina chips WG 12 for whole PBMC/SFMC population, T cells, B cells and monocytes. Some of the significant genes were validated by qRT-PCR.
Genes up regulated
Genes down regulated
Number of dysregulated pathways [total (significant)]
Pathways of immunological relevance
EB vs EF
Cell adhesion, IgA production, antigen processing, lysosomal processing
CBMO vs EBMO
Cytokine signaling, TLR signaling, antigen presentation, chemokines signaling
EBMO vs EFMO
Complement cascade, cytokine signaling, antigen presentation
Monocyte probably play a major role in pathogenesis of JIA-ERA and TLR signalling may be the pathway involved.
Disclosure of interest
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.