- Research article
- Open Access
Histologic effects of mandibular protrusion splints in antigen-induced TMJ arthritis in rabbits
© The Author(s). 2017
- Received: 10 December 2016
- Accepted: 4 April 2017
- Published: 13 April 2017
Although it is common clinical practice to treat children with Juvenile Idiopathic Arthritis (JIA) with functional appliances, the scientific evidence for this is limited. The aim of this study was to study the histologic effects of mandibular protrusion splints in temporomandibular joint (TMJ) arthritis in rabbits.
Twenty-eight ten-week old New Zealand white rabbits were randomly divided into four groups: AO (TMJ arthritis, no splint), AS (TMJ arthritis, mandibular splint advancement), OS (no arthritis, mandibular splint advancement) and OO (no arthritis, no splint). TMJ arthritis was induced in the groups AO and AS; 1 week later mandibular protrusion splints were placed on the upper incisors of the AS and OS animals. After 60 days the animals were sacrificed and a semiquantitative histologic evaluation of each TMJ was carried out to analyze the amount of inflammation and bone modeling.
AO and AS animals had a higher inflammation score (AO = 1.3; AS = 1.8) than the non-arthritis groups (OO = 0.6; OS = 0.4). Whereas in the untreated control (OO) the amount of apposition and resorption was almost in balance (+1), OS animals displayed significantly more apposition (+9) and AO animals significantly more resorption (−3) than the untreated control. Arthritis animals with protrusion appliances (AS), however, had remarkably more bone apposition (+3) than resorption, indicating a similar bony reaction as in healthy animals, although reduced in extent.
Mandibular advancement in rabbits with TMJ arthritis is possible without detrimental histologic reactions and appears to partially compensate for the bone loss seen in rabbits with TMJ arthritis but without protrusion splints.
- TMJ morphology
- Functional mandibular advancement
Juvenile idiopathic arthritis (JIA) is one of the most common chronic diseases in childhood, with a reported prevalence of 1 in 1000 children . The prevalence of clinically detectable temporomandibular joint (TMJ) involvement varies between 38 and 72%, depending on the diagnostic method used and the JIA-subtype examined [2–8]. This TMJ inflammation may cause significant limitations in sagittal and vertical mandibular growth, conditionally resulting in severe micrognathia and anterior open bites with marked esthetic and functional restrictions [2, 3, 9–19].
Overall, the evidence on orthodontic treatment principles for JIA-children with TMJ involvement is low. However, there is limited evidence that dentofacial orthopedic treatment using functional appliances can improve mandibular retrognathia and reduce pain in adolescent JIA-patients . It has also been demonstrated that in cases with unilateral TMJ involvement, resulting in an asymmetrical mandibular growth, functional appliance treatment can reduce these asymmetries . Thus, it appears to be clinically possible to enhance growth in JIA children with TMJ involvement to a certain extent and partially compensate for the growth limitations caused by the inflammatory process [22–24].
Nevertheless, some authors advise not to “strain” the TMJ, as would be the case during mandibular advancement procedures, because they fear accelerated condylar destruction of the TMJ as a result of an increased bone turnover rate . Considering the results of basic cell research, however, these fears seem unnecessary, since numerous in vitro studies have shown an anti-inflammatory effect of tensile strain on inflamed chondrocytes [26–29]. Furthermore, condyles have an exceptional potential for regeneration and remodelling, to an extent that even in growing rats, where the condyles had been experimentally resected, growth could be enhanced through mandibular advancement .
For healthy individuals, it is well known that treatment with functional appliances induces bone modelling in the TMJ [31, 32]. Furthermore, it has been shown that patients treated with a Herbst appliance displayed a certain amount of regeneration of arthrotic TMJ-lesions [33, 34]. Therefore, it was assumed that also children with an arthritic TMJ involvement could benefit from functional mandibular advancement with a fixed functional appliance. Since, however, in JIA children many cofactors can influence the reaction (disease duration, general medication, degree of TMJ involvement, age, subtype etc.), making it extremely difficult, if not impossible, to get a patient cohort with a comparable situation, it was decided to analyse the treatment effects in an animal model.
The aim of this animal study was to analyse the histologic effects of mandibular protrusion appliances in rabbits with antigen induced arthritis of the temporomandibular joint. The null hypothesis was that there would be no histologic differences concerning inflammation or bone remodelling in the TMJs of rabbits with an antigen induced arthritis between animals treated with or without mandibular protrusion appliances.
Twenty-eight ten-week old New Zealand white rabbits (Oryctolagus cuniculus) were housed at the central laboratory animal facilities of the University of Giessen, Germany and had constant access to food and water. To ensure that the protrusion appliances would not hinder the animals from eating, the food (dried pellets) was moistened for all animals. Animal welfare was supervised daily by weight control as well as evaluation of food and water intake. All procedures were approved by the government ethical committee for animal welfare in Giessen, Germany (71/2008).
Randomized distribution of the 28 New Zealand White rabbits into the different experimental groups (A0, AS, 0S, 00)
AS (n = 7)
A0 (n = 7)
No protrusion splint
0S (n = 7)
00 (n = 7)
Criteria for the semiquantitative scoring of inflammation and bone modelling
slightly more resorption than apposition (catabolic situation)
amount of resorption equals apposition (balanced situation)
slightly more apposition than resorption (anabolic situation)
moderately more apposition than resorption (anabolic situation)
Statistical calculations were done with SPSS Statistics 22 (SPSS Inc. an IBM Company, Chicago, IL). All tests were performed two sided with a significance level of 5%. An alpha adjustment for multiple testing was not applied, and the results were interpreted accordingly as being explorative. Ordinally scaled histological values were displayed in absolute and percent frequencies. Groups or sides were compared regarding these values in contingency tables and tested for dependence with chi-square trend test.
Unfortunately, two animals of the A0 group were lost during induction of the TMJ arthritis: the first due to respiratory arrest under general anesthesia, the second due to an anaphylactic shock. All others completed the trial without complications with a normal weight development.
Inflammation scores (absolute) and indexes (relative) for the four experimental groups (00, 0S, A0, AS): 0 = none, 1 = slight, 2 = moderate, 3 = severe
Analyzable joints (n)
Inflammation score (absolute)
Inflammation index (relative)
Limitation of the study
To our knowledge, no validated method for a quantitative analysis of histologic changes in the rabbit TMJ has been described, although other authors have performed such an evaluation using varying methods in rabbits and even rats [36–38]. Despite the fact that two very experienced veterinary pathologists performed the present sample preparation (KK) and histological evaluation (KK and KS), it was not possible to obtain the exact same sectional plane in all samples, primarily due to interindividual morphologic differences. Thus, although originally planned, we eventually refrained from undertaking a quantitative analysis. Concerning the reliability of the alternative semiquantitative measurements is was found that both pathologists scored the inflammation and bone resorption very similarly whereas the amount of new bone formation was the main object of discussion. Here in 11 of the 52 samples an initial disagreement concerning the amount of bone apposition was evident, which, however, was agreed upon without problems. Furthermore, it has to be remarked that retrospectively it would have been nice to have had pre- and posttreatment CBCTs to document mandibular growth. Unfortunately this idea did not come to our mind before conducting the trial so that this data is not available. An expected complication, the overgrowth of the incisors, was not observed. It appeared as if the lower incisors showed a more or less normal wear at the end of the observation period, the protrusion appliances were still in place in all animals and the upper incisors were of normal length.
As expected, the inflammatory markers were higher in the arthritis groups (AS and A0) than in the non arthritis groups (0S and 00). Nevertheless, it has to be remarked that the untreated control group also exhibited a certain amount of inflammatory cells. This might appear surprising, but due to the fact that only growing animals, who constantly exhibit a certain amount of bone remodelling, were analysed, the presence of inflammatory cells is a physiological circumstance. When comparing 00 to 0S animals, slightly less inflammatory markers were present in the group with protrusion appliances. Comparing A0 to AS animals, however, the group with protrusion appliances exhibited more signs of inflammation. This relative increase of inflammation is assumingly an addition of the TMJ arthritis to the reaction observed during mandibular advancement. It has been observed that also systemically healthy dentofacial orthopedic patients (Herbst appliance and Andresen activator) exhibit an inflammatory reaction during condylar adaptation [34, 39]. This is a physiological reaction upon mandibular advancement and represents the preliminary stage to new bone formation. It appears reasonable, that this adaptation is slower in arthritis patients than in healthy subjects, since the “physiological” inflammation adds up on the antigen-induced TMJ arthritis. It might well be that bone modelling due to mandibular advancement was not completed at the time of sacrifice and thus the duration of the trial was possibly too short. This becomes particularly likely as none of the other parameters indicated a pathologic process (not more bone resorption, not more joint destruction). Furthermore it has to be remarked that although the AS animals did have a tendency to higher inflammatory markers than the A0 animals, this difference could not be statistically verified.
All rabbits showed a certain amount of bone modelling, which was expected, as all were growing animals. Whereas a more or less balanced amount of apposition and resorption was found for the untreated control group (00), the A0 animals exhibited a negative bone turnover (bone resorption > bone apposition). This bony resorption is most likely due to the induced arthritis and can often be verified in JIA-children [12, 13, 40, 41] where condylar erosions or flattening are found in MRIs and in extreme cases, even in orthopantomograms or ultrasound images [42, 43]. Compared to the A0 group, AS animals had significantly less bone resorption and in one case even more apposition than resorption. This positive effect is most likely due to the functional mandibular advancement. Although it is commonly known that functional therapy can induce bone apposition in the TMJ, this has so far not been evaluated in JIA patients. Recently Stoustrup et al.  described a positive effect of distraction appliances on JIA-caused facial asymmetries. Whether or not this positive effect is due to a skeletal reaction in the TMJ or due to a muscular adaptation can not be verified in a clinical study. The present data, however, suggest that a positive skeletal reaction can be obtained by functional orthopedic treatment even in cases with TMJ arthritis.
It was striking that if degenerative condylar changes occurred, they were always located in the anterior cranial region of the condyle, whereas the posterior areas were unaffected, indicating that mandibular growth was not restrained in a balanced way. Consequently the posterior condylar growth would continue normally, whereas the anterior-cranial growth direction would be restrained. This could lead to a posterior mandibular rotation (counter clockwise), resulting in an opening of the mandibular plane angle, as already described by Björk and Skieller [44, 45] in a case with an untreated JIA.
Limiting it has to be remarked that the results of this research can only partly be transferred to the clinical treatment of JIA children. Primarily it has to be considered that in this study an acute and early TMJ arthritis situation was analyzed, since the appliances were placed 1 week after the ovalbumin injection. It might well be that the amount of bone destruction caused in the TMJ during this 1 week would have been less if the protrusion appliance would have been inserted directly after the ovalbumin injection. However, in the clinical situation most JIA children that are being treated with functional appliances have already had an inflammation in the TMJ. Thus the fact that the appliances were placed 1 week after the TMJ arthritis has caused some degree of damage is closer to clinical reality. With the present study design, however, it remains unclear whether or not the mandibular protrusion has any preventive effects during inflammatory flares. Furthermore, the rabbits did not receive any kind of systemic medication, which would normally be a cofactor in the clinical situation in JIA children. In JIA patient treatment a large variety of drugs is applied (NSARs, glucocorticoids, DMARDs, biologica) [46, 47], which all can influence the course of the disease and whose positive or negative interactions with functional mandibular advancement are completely unknown. This should be evaluated in a further study. Additionally, in the present study only one single ovalbumin injection was performed in the TMJ which simulates the acute arthritis attack. This acute stage, however, was not reactivated through further reinjections and of course this might have had an impact on the findings after 60 days. However, it also has to be remarked that JIA children seldom have an acute TMJ inflammation over such a long time period and that also here the acute stage often becomes chronic, but of course this all should be evaluated in further studies.
Despite a higher inflammatory reaction in arthritic joints, the insertion of a protrusion appliance in rabbits with an antigen induced TMJ arthritis lead to more bone apposition than in functionally untreated animals. Thus, the null hypothesis had to be rejected. Due to the relative small sample size and the large interindividual range of reactions, statistical significances could not be verified for all parameters. Since this study was performed on rabbits, the results can only be trend-setting as their transferability to JIA children is limited.
Juvenile idiopathic arthritis
TMJ arthritis, no mandibular protrusion splint
TMJ arthritis, mandibular protrusion splint
no TMJ arthritis, no mandibular protrusion splint
no TMJ arthritis, mandibular protrusion splint
This research was supported by a grant from the Medical faculty of the Justus-Liebig-University Giessen, Germany (grant number: 600 80 169) awarded to JvB.
Availability of data and materials
JvB planned the study, carried out the experiments (sensitation, placement of protrusion appliances) and drafted the manuscript. KK and KS performed the histologic sample preparation, evaluation and interpretation. DZ is the head of the animal facilities who performed the intraarticular TMJ injections and sacrificed the animals. SR carried out the experiments (sensitation, placement of protrusion appliances) and helped to revise the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
The protocol was approved by the the government ethical committee for animal welfare in Giessen, Germany (71/2008).
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- Minden K. Juvenile idiopatische arthritis. In: Wagner N, Dannecker G, editors. Pädiatrische rheumatologie. 12th ed. Heidelberg: Springer Publishing Company; 2007. p. 177–81.Google Scholar
- Karhulahti T, Ylijoki H, Ronning O. Mandibular condyle lesions related to age at onset and subtypes of juvenile rheumatoid arthritis in 15-year-old children. Scand J Dent Res. 1993;101:332–8.PubMedGoogle Scholar
- Kreiborg S, Bakke M, Kirkeby S, Michler L, Vedtofte P, Seidler B, et al. Facial growth and oral function in a cases of juvenile rheumatoid arthritis during an 8-year period. Eur J Orthod. 1990;12:119–34.View ArticlePubMedGoogle Scholar
- Olson L, Eckerdal O, Hallonsten AL, Helkimo M, Koch G, Gäre BA. Craniomandibular function in juvenile chronic arthritis. A clinical and radiographic study. Swed Dent J. 1991;15:71–83.PubMedGoogle Scholar
- Pearson MH, Ronning O. Lesions of the mandibular condyle in juvenile chronic arthritis. Br J Orthod. 1996;23:49–56.View ArticlePubMedGoogle Scholar
- Schwarzer CL, Bumann A, Küster RM. Frühe Kiefergelenkbefunde bei Kindern mit Juveniler Chronischer Arthritis auf der Grundlage manueller Untersuchungstechniken. Inf Orthod Kieferorthop. 1995;27:549–63.Google Scholar
- Twilt M, Mobers SM, Arends LR, ten Cate R, van Suijlekom-Smit L. Temporomandibular involvement in juvenile idiopathic arthritis. J Rheumatol. 2004;31:1418–22.PubMedGoogle Scholar
- Wenneberg B, Kjellberg H, Kiliaridis S. Bite force and temporomandibular disorder in juvenile chronic arthritis. J Oral Rehabil. 1995;22:633–41.View ArticlePubMedGoogle Scholar
- Farronato G, Carletti V, Maspero C, Farronato D, Giannini L, Bellintani C. Craniofacial growth in children affected by JIA involving the TMJ: functional therapy. J Clin Pediatr Dent. 2009;33:351–7.View ArticlePubMedGoogle Scholar
- Grosfeld O. The orthodontist in the team-treatment for children with rheumatoid arthritis. Eur J Orthod. 1989;11:120–4.View ArticlePubMedGoogle Scholar
- Hanna VE, Rider SF, Moore TL, Wilson VK, Osborn TG, Rotskoff KS, Johnston Jr LE. Effects of systemic onset juvenile rheumatoid arthritis on facial morphology and temporomandibular joint form and function. J Rheumatol. 1996;23:155–8.PubMedGoogle Scholar
- Hu YS, Schneiderman ED. The temporomandibular joint in juvenile rheumatoid arthritis: I. Computed tomographic findings. Pediatr Dent. 1995;17:46–53.PubMedGoogle Scholar
- Hu YS, Schneiderman ED, Harper RP. The temporomandibular joint in juvenile rheumatoid arthritis: Part II. Relations between computed tomographic and clinical findings. Pediatr Dent. 1996;18:312–9.PubMedGoogle Scholar
- Jakobsson SO, Paulin G. The influence of activator treatment on skeletal growth in Angle Class II: 1 cases. A roentgenocephalometric study. Eur J Orthod. 1990;12(2):174–84.View ArticlePubMedGoogle Scholar
- Kitai N, Kreiborg S, Makke M, Paulsen HU, Møller E, Darvann TA, Pedersen H, Takada K. Three-dimensional magnetic resonance image of the mandible and masticatory muscles in a case of juvenile chronic arthritis treated with the herbst appliance. Angle Orthod. 2002;72:81–7.PubMedGoogle Scholar
- Kjellberg H, Fasth A, Kiliaridis S, Wenneberg B, Thilander B. Craniofacial structure in children with juvenile chronic arthritis (JCA) compared with healthy children with ideal or postnormal occlusion. Am J Orthod Dentofac Orthop. 1995;107:67–78.View ArticleGoogle Scholar
- Kjellberg H, Kiliaridis S, Thilander B. Dentofacial growth in orthodontically treated and untreated children with juvenile chronic arthritis (JCA). a comparison with angle class II division 1 subjects. Eur J Orthod. 1995;17:357–73.View ArticlePubMedGoogle Scholar
- Kjellberg H. Craniofacial growth in juvenile chronic arthritis. Acta Odont Scand. 1998;56:360–5.View ArticlePubMedGoogle Scholar
- Peltomäki T, Kreiborg S, Pedersen TK, Ogaard B. Craniofacial growth and dento-alveoalr development in juvenile idiopathic arthritis patients. Semin Orthod. 2015;21:84–93.View ArticleGoogle Scholar
- von Bremen J, Ruf S. Orthodontic and dentofacial orthopedic management of juvenile idiopathic arthritis: a systematic review of the literature. Orthod Craniofac Res. 2011;14(3):107–15.View ArticleGoogle Scholar
- Stoustrup P, Küseler A, Kristensen KD, Herlin T, Pedersen TK. Orthopaedic appliance treatment can reduce mandibular asymmetry caused by unilateral temporomandibular involvement in juvenile idiopathic arthritis. Eur J Orthod. 2013;35(2):191–8.View ArticlePubMedGoogle Scholar
- Pedersen TK, Grønhøj J, Melsen B, Herlin T. Condylar condition and mandibular growth during early functional treatment of children with juvenile chronic arthritis. Eur J Orthod. 1995;17(5):385–94.View ArticlePubMedGoogle Scholar
- Pedersen TK, Norholt SE. Early orthopedic treatment and mandibular growth of children with temporomandibular joint abnormalities. Semin Orthod. 2011;17:235–45.View ArticleGoogle Scholar
- Pedersen TK, Verna C. Functional and orthopaedic treatment in developing dentofacial growth deviation in juvenile idiopathic arthritis. Semin Orthod. 2015;21:134–9.View ArticleGoogle Scholar
- Proffit WR, Turvey TA. Dentofacial asymmetry. In: Proffit WR, White RP, Sarver DM, editors. Contemporary treatment of dentofacial deformity. St. Louis: Elsevier; 2003. p. 600.Google Scholar
- Gassner R, Buckley MJ, Georgescu H, Studer R, Stefanovich-Racic M, Piesco NP, Evans CH, Agarwal S. Cyclic tensile stress exerts antiinflammatory actions on chondrocytes by inhibiting inducible nitric oxide synthase. J Immunol. 1999;163(4):2187–92.PubMedPubMed CentralGoogle Scholar
- Agarwal S, Long P, Gassner R, Piesco NP, Buckley MJ. Cyclic tensile strain suppresses catabolic effects of interleukin-1beta in fibrochondrocytes from the temporomandibular joint. Arthritis Rheum. 2001;44(3):608–17.View ArticlePubMedPubMed CentralGoogle Scholar
- Deschner J, Rath-Deschner B. Agarwal S Regulation of matrix metalloproteinase expression by dynamic tensile strain in rat fibrochondrocytes. Osteoarthr Cartil. 2006;14(3):264–72.View ArticlePubMedGoogle Scholar
- Ferretti M, Madhavan S, Deschner J, Rath-Deschner B, Wypasek E, Agarwal S. Dynamic biophysical strain modulates proinflammatory gene induction in meniscal fibrochondrocytes. Am J Physiol Cell Physiol. 2006;290(6):C1610–5.View ArticlePubMedPubMed CentralGoogle Scholar
- Spyropoulos MN, Tsolakis AI. Altered mandibular function and prevention of skeletal asymmetries after unilateral condylectomie in rats. Eur J Orthod. 1997;19:211–8.View ArticlePubMedGoogle Scholar
- Ruf S, Pancherz H. Temporomandibular joint growth adaptation in Herbst treatment: a prospective magnetic resonance imaging and cephalometric roentgenographic study. Eur J Orthod. 1998;20:385–8.View ArticleGoogle Scholar
- Ruf S, Pancherz H. Temporomandibular joint remodelling in adolescents and young adults during Herbst treatment: A prospective longitudinal magnetic resonance imaging and cephalometric investigation. Am J Orthod Dentofac Orthop. 1999;115:607–18.View ArticleGoogle Scholar
- Aidar LA, Abrahão M, Yamashita HK, Dominguez GC. Morphological changes of condyles and Helkimo clinical dysfunction index in patients treated with Herbst--orthodontic appliance. Braz Dent J. 2013;24(4):313–21.View ArticlePubMedGoogle Scholar
- Ruf S, Pancherz H. Does bite-jumping damage the TMJ? a prospective longitudinal clinical and MRI study of herbst patients. Angle Orthod. 2000;70:183–99.PubMedGoogle Scholar
- Kapila S, Lee C, Tavakkoli-Jou MR, Miller AJ, Richards DW. Development and histologic characterizations of an animal model of antigen-induced arthritis of the juvenile rabbit temporomandibular joint. J Dent Res. 1995;74(12):1870–9.View ArticlePubMedGoogle Scholar
- Rabie AB, Zhao Z, Shen G, Hägg EU, Robinson W. Osteogenesis in the glenoid fossa in response to mandibular advancement. Am J Orthod Dentofac Orthop. 2001;119:390–400.View ArticleGoogle Scholar
- Shen G, Rabie AB, Zhao ZH, Kaluarachchi K. Forward deviation of the mandibular condyle enhances endochondral ossification of condylar cartilage indicated by increased expression of type X collagen. Arch Oral Biol. 2006;51(4):315–24.View ArticlePubMedGoogle Scholar
- Wu MJ, Zhan J, Gu ZY. Time course of expression of bcl-2 and bax in rabbit condylar chondrocytes following forward mandibular positioning. Angle Orthod. 2008;78(3):453–9.View ArticlePubMedGoogle Scholar
- Ruf S, Wüsten B, Pancherz H. Temporomandibular joint effects of activator treatment: a prospective longitudinal magnetic resonance imaging and clinical study. Angle Orthod. 2002;72:527–40.PubMedGoogle Scholar
- Bayar N, Kara SA, Keles I, et al. Temporomandibular joint involvement in rheumatoid arthritis: a radiological and clinical study. Cranio. 2002;20:105–10.View ArticlePubMedGoogle Scholar
- Küseler A, Pedersen TK, Gelineck J, et al. A 2 year follow up study of enhanced magnetic resonance imaging and clinical examination of the temporomandibular joint in children with juvenile chronic arthritis. J Rheumatol. 2005;32:162–9.PubMedGoogle Scholar
- Assaf AT, Kahl-Nieke B, Feddersen J, Habermann CR. Is high-resolution ultrasonography suitable for the detection of temporomandibular joint involvement in children with juvenile idiopathic arthritis? Dentomaxillofac Radiol. 2013;42(3):20110379.View ArticlePubMedPubMed CentralGoogle Scholar
- Pedersen TK, Jensen JJ, Melsen B, Herlin T. Resorption of the temporomandibular condylar bone according to subtypes of juvenile chronic arthritis. J Rheumatol. 2001;28(9):2109–15.PubMedGoogle Scholar
- Björk A, Skieller V. Normal and abnormal growth of the mandible. A synthesis of longitudinal cephalometric implant studies over a period of 25 years. Eur J Orthod. 1983;5:1–46.View ArticlePubMedGoogle Scholar
- Björk A, Skieller V. Contrasting mandibular growth and facial development in long face syndrome, juvenile rheumatoid polyarthritis, and mandibulofacial dysostosis. J Craniofac Genet Dev Biol Suppl. 1985;1:127–38.PubMedGoogle Scholar
- Stoll ML, Cron RQ. Treatment of juvenile idiopathic arthritis in the biologic age. Rheum Dis Clin North Am. 2013;39(4):751–66.View ArticlePubMedGoogle Scholar
- Stoll ML, Cron RQ, Saureanmann RK. Systemic and intra-articular anti-inflammatory therapy of temporomandibular joint arthritis in children with juvenile idiopathic arthritis. Semin Orthod. 2015;21:125–33.View ArticleGoogle Scholar