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Characterization of B cells in synovial fluid and tissue from patients with JIA
Pediatric Rheumatology volume 6, Article number: P21 (2008)
The nature of B cell subsets infiltrating the synovial membrane from JIA patients is poorly defined. To this aim we performed an immunophenotypic and functional characterization of B cells in JIA patients.
MNC from synovial fluid (SF) and paired peripheral blood (PB) from 25 JIA patients and 20 age-matched controls were analyzed with multi-colour flow cytometry.
SF B cells were found to be significantly enriched in CD27+ switch memory (sm) 1 cells and in the recently identified isotype class switch memory (CD19+CD27-IgG+IgA+) B cells (sm2) compared to paired and healthy PB (P < 0.0001). CCR5, CCR8, and CCR9 expression was significantly higher on SF sm1 and sm2 B cells than on correspondent paired PB B cells (P < 0.001). Naïve (IgD+, CD27-) B cells were significantly reduced in SF compared to paired and control PB (P < 0.0001). Similarly, transitional B cells (CD19+CD24highCD38highIgMhighIgDhigh) were significantly less numerous in SF than in paired PB from JIA patients (P < 0.0001).
Plasma blasts were significantly enriched in SF than in paired PB (P = 0.005). ELISPOT experiments showed significantly higher proportions of CD19+ IgG secreting cells in SF vs paired JIA PB (P = 0.028). Histological analysis of synovial tissue sections demonstrated the presence of lymphoid aggregates containing clusters of CD20+ cells surrounded by CD138+plasmablasts/plasmacells producing predominantly IgG.
These findings support a model whereby memory B cells are selectively attracted through chemokine gradients to the inflamed joints of JIA patients and differentiate locally into plasmablasts/plasmacells in the absence of ectopic follicular structures.
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Ferlito, F., Corcione, A., Traggiai, E. et al. Characterization of B cells in synovial fluid and tissue from patients with JIA. Pediatr Rheumatol 6, P21 (2008) doi:10.1186/1546-0096-6-S1-P21
- Synovial Fluid
- Synovial Tissue
- Inflame Joint
- CCR9 Expression
- Switch Memory