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High membrane expression of CD163 by bone marrow cells is not a specific marker of macrophage activation syndrome (MAS)

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Background

MAS is a life-threatening complication seen predominantly in children with SoJIA, often difficult to recognize because specific diagnostic criteria for MAS have not yet been devised. MAS is characterized by an overwhelming inflammatory reaction driven by excessive T cell expansion and hemophagocytic macrophages. Preliminary studies suggest that high sIL-2R α serum levels and sCD163 might be useful as diagnostic markers for MAS. This study assesses the expression of CD163 and CD68 in bone marrow cells of patients with MAS and especially in those with SoJIA who developed MAS.

Methods

Thirteen bone marrow biopsies (BMB) were performed, 7 on patients with MAS secondary to SoJIA, 6 with virus-induced HLH. Fifteen BMB controls were included. Immunohistochemical staining was performed using monoclonal antibodies directed against CD163 and CD68.

Results

CD163 immunoreactivity was characterized by a strong, granular cytoplasmic or cytoplasmic and membrane staining pattern. The macrophage marker CD68 showed a granular cytoplasmic pattern. Increased numbers of macrophages were observed in the bone marrow of all MAS and HLH samples. CD163 expression in macrophages was brighter than that of CD68. CD163 expression was similar in MAS associated with SoJIA and in virus induced HLH.

Conclusion

Our data demonstrate that CD163 in BMB is restricted to the monocyte/macrophage lineage but is not specific for MAS. Furthermore CD163 is similarly expressed by macrophages from biopsies of both SoJIA and HLH patients. This antibody is a marker of activated macrophages but cannot differentiate patients with MAS from patients with other diseases of the macrophage lineage.

References

  1. 1.

    Bleesing J, Prada A, Siegel DM, Villanueva J, Olson J, Ilowite NT, Brunner HI, Griffin T, Graham TB, Sherry DD, Passo MH, Ramanan AV, Filipovich A, Grom AA: The Diagnostic Significance of Soluble CD163 and Soluble Interleukin-2 Receptor – Chain in Macrophage Activation Syndrome and Untreated New-Onset Systemic Juvenile Idiopathic Arthritis. Arthritis Rheum. 2007, 56: 965-971. 10.1002/art.22416.

  2. 2.

    Schaer DJ, Schleiffenbaum B, Kurrer M, Imhof A, Bächli E, Fehr J, Moller HJ, Moestrup SK, Schaffner A: Soluble hemoglobin-haptoglobin scavengerreceptor CD163 as a lineage-specific marker in the reactive hemophagocytic syndrome. Eur J Haematol. 2005, 74: 6-10. 10.1111/j.1600-0609.2004.00318.x.

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Author information

Correspondence to C Bracaglia.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Bracaglia, C., Devito, R., Insalaco, A. et al. High membrane expression of CD163 by bone marrow cells is not a specific marker of macrophage activation syndrome (MAS). Pediatr Rheumatol 6, P17 (2008) doi:10.1186/1546-0096-6-S1-P17

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Keywords

  • CD163 Expression
  • Bone Marrow Cell
  • Bone Marrow Biopsy
  • Marker CD68
  • Membrane Staining