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Fig. 1 | Pediatric Rheumatology

Fig. 1

From: Blood brain barrier permeability and astrocyte-derived extracellular vesicles in children with juvenile idiopathic arthritis: a cross-sectional study

Fig. 1

Representative plots of flow cytometric analysis of extracellular vesicles (EVs). (A) Dot-plot of Nano fluorescent Yellow Particles used to determine the EV gate. (B) Gating strategy for all EVs based on size and complexity. (C1-4) Beside size and complexity, samples were controlled for single gating (C1 and C2). Unstained EVs, Iso-type controls, single fluorochrome stained EVs, and EVs stained as fluorescence-minus-one (FMO) were used to fine tune the panel (C4). Representative sample of an JIA patient demonstrating EVs labeled with anti-Aquaporin-4 Dylight 488 and anti-GFAP Dylight 755

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Pediatric Rheumatology

ISSN: 1546-0096