Table 1 Primers used for qPCR experiments
From: Potential regulatory role of the Nrf2/HMGB1/TLR4/NF-κB signaling pathway in lupus nephritis
Primer name | Primer sequence | Primer length (nt) | Product length (bp) | Annealing temperature (℃) |
|---|---|---|---|---|
Nrf2 F | CTTTAGTCAGCGACAGAAGGAC | 22 | 227 | 47.8 |
Nrf2 R | AGGCATCTTGTTTGGGAATGTG | 22 | ||
HMGB1 F | GGCGAGCATCCTGGCTTATC | 20 | 86 | 60.1 |
HMGB1 R | GGCTGCTTGTCATCTGCTG | 19 | ||
TLR4 F | GAAGCTTGAATCCCTGCATAGAGGT | 25 | 249 | 49.0 |
TLR4 R | AGTTTGAGAGGTGGTGTAAGCC | 22 | ||
NF-κB F | CACGAGGCTCCTTTTCTCAA | 20 | 270 | 58.3 |
NF-κB R | GGGGTTCAGTTGGTCCATTG | 20 | ||
β-actin F | AGGGAAATCGTGCGTGAC | 18 | 192 | 58.0 |
β-actin R | CATACCCAAGAAGGAAGGCT | 20 |