MicroRNA-27a-3p enhances the inflammatory phenotype of Juvenile Idiopathic Arthritis fibroblast-like synoviocytes

Table 3 FLS pro-inflammatory potential remained intact after miR-27a-3p transfection

Readout		Untransfected (n = 5)			miR-NC (n = 8)			miR-27a-3p (n = 8)
Readout		Medium	SF	Fold Change	Medium	SF	Fold Change	Medium	SF	Fold Change
mRNA	IL6	13.0 ± 5.4	*226.1 ± 62.4	26.9 ± 9.6	16.1 ± 3.1	*370.5 ± 130.9	21.5 ± 5.0	30.1 ± 10.7	**421.5 ± 119.8	20.7 ± 6.9
	IL8	n.d.	n.d.	n.d.	5.4 ± 1.2	**16.8 ± 2.5	4.4 ± 1.1	7.1 ± 1.8	*70.0 ± 50.5	14.6 ± 11.3
	CCL2	n.d.	n.d.	n.d.	3.2 ± 0.3	**35.0 ± 7.3	10.8 ± 1.9	6.4 ± 2.8	**41.9 ± 9.2	9.1 ± 1.4
	MMP3	7.0 ± 3.0	89.1 ± 42.3	30.7 ± 20.3	7.3 ± 1.6	*61.2 ± 18.5	9.5 ± 3.1	12.4 ± 6.1	**3094.1 ± 3039.1	63.4 ± 55.0
Protein	IL-6	n.d.	n.d.	n.d.	41.9 ± 8.1	***632.5 ± 38.9	79.1 ± 64.0	49.6 ± 10.7	****654.6 ± 35.0	70.1 ± 56.5
	IL-8	n.d.	n.d.	n.d.	13.7 ± 2.4	***255.1 ± 32.2	21.7 ± 4.1	20.3 ± 1.7	**251.5 ± 38.5	13.3 ± 2.8
	CCL2	n.d.	n.d.	n.d.	972.7 ± 220.6	***5790.8 ± 681.6	6.7 ± 0.7	942.1 ± 198.4	***5570.3 ± 818.1	6.4 ± 0.7

Data are presented as relative expression values for mRNA and pg/mL for protein. Fold changes were calculated as SF compared to medium. Data are presented as mean ± SEM. *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, when comparing medium to SF within cells of the same transfection status. SF, synovial fluid

ISSN: 1546-0096