MicroRNA-27a-3p enhances the inflammatory phenotype of Juvenile Idiopathic Arthritis fibroblast-like synoviocytes

Table 2 Primers and annealing temperatures

Target	Primer Sequence or Commercial Product	Supplier	Annealing Temperature (°C)
miR-27a-3p	TTCACAGTGGCTAAGTTCCGC	Invitrogen	55
miR-93-5p	GCAAAGTGCTGTTCGTG	Invitrogen	55
miR-191-5p	CGCGCAACGGAATCCCA	Invitrogen	55
CCL2	PrimePCR assay	Bio-Rad	60
CXCL8	PrimePCR assay	Bio-Rad	53
GAPDH	PrimePCR assay	Bio-Rad	60
HPRT1	PrimePCR assay	Bio-Rad	60
IL6	RT² qPCR Primer Assay	Qiagen	60
IL17A	PrimePCR assay	Bio-Rad	53
MMP3	Sense: 5’AGTCTTCCAATCCTACTGTTGCT Antisense: 5’TCCCCGTCACCTCCAATCC	Invitrogen	53

CCL2 C-C motif chemokine ligand 2, CXCL8 C-X-C motif chemokine ligand 8, GAPDH Glyceraldehyde 3-phosphate dehydrogenase, HPRT1 Hypoxanthine phosphoribosyltransferase 1, MMP3 Matrix metalloproteinase 3, TNFA Tumor necrosis factor alpha

ISSN: 1546-0096