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Fig. 3 | Pediatric Rheumatology

Fig. 3

From: MicroRNA-27a-3p enhances the inflammatory phenotype of Juvenile Idiopathic Arthritis fibroblast-like synoviocytes

Fig. 3

Transfection with miR-27a-3p enhances FLS cytokine secretion. FLS were transfected with either miR-27a-3p mimic or miR-NC, then stimulated with pooled JIA synovial fluid (SF), or were left unstimulated. A Expression of the indicated cytokine genes was quantified using RT-qPCR, normalized to reference genes HPRT1 and GAPDH. Graphs show individual paired samples (n = 8). B Secretion of IL-6, CXCL8/IL-8, and CCL2 protein in culture supernatants was quantified by ELISA. Graphs show individual paired samples (n = 6–8). *p < 0.05

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Pediatric Rheumatology

ISSN: 1546-0096