Fig. 3

Paired analysis of active and inactive sJIA. A Comparison of protein levels in paired samples from nine patients during active and inactive disease phases. Each symbol represents one sample, and the lines link the paired samples. B Hierarchical clustering analysis shows the grouping of active and inactive sJIA based on the eleven significantly different proteins. In the heat map, rows were centered and unit variance scaling was applied to rows; both rows and columns were clustered using maximum distance and average linkage. C Principle component analysis of the three groups. The confidence level of the ellipses is 0.90. D Receiver operating characteristic (ROC) curves examining the predictive performance of the eleven proteins for distinguishing active sJIA (n = 14) in reference to inactive sJIA (n = 16) in the cross-sectional analysis. E Area under the curve (AUC) and corresponding 95% CI for each measure. Statistics: A two-way repeat-measurement ANOVA with correction of multiple comparison by controlling the False Discovery Rate (FDR) of 5% via two-stage step-up method of Benjamini, Krieger and Yekutieli, * p < 0.05, ** p < 0.01, **** p < 0.0001, (E) the 95% confidence interval were calculated by hybrid Wilson/Brown method