Fig. 1

Distribution of the different subgroups based on detected inflammation-associated proteins. A Principal component analysis (PCA) of the three groups based on all included 69 proteins. Confidence level of the ellipses is 0.90. B Random forest analysis resulted in a predictive accuracy of 90.6%. The factor importance plot displays the top contributing proteins with importance higher than 0.01. C Comparative analysis of the proteins with top importance in (B) revealed significant difference among at least one of the three comparisons. Bars represent mean ± standard deviation. D Hierarchical clustering analysis based on the 10 proteins in (C) showing the grouping among active sJIA, inactive sJIA and controls. Unit variance scaling was applied to rows; both rows and columns were clustered using correlation distance and average linkage. E PCA of the three groups based on the 10 proteins shown in (C). The confidence level of the ellipses is 0.90. Statistics: ordinary two-way ANOVA with correction of multiple comparison by controlling the False Discovery Rate (FDR) of 5% via two-stage step-up method of Benjamini, Krieger and Yekutieli, * p < 0.05, ** p < 0.01, *** p < 0.001