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Fig. 5 | Pediatric Rheumatology

Fig. 5

From: Joint contractures responsive to immunosuppressive therapy in a girl with childhood‐onset systemic sclerosis double‐seropositive for rare anti‐nucleolar autoantibodies: a case report

Fig. 5

Identification of SSc-related autoantibodies. a RNA immunoprecipitation assay for our patient’s serum. The immunoprecipitates obtained by serum samples (our patient’s serum, anti-Th/To-positive reference serum, and anti-signal recognition particle [SRP]-positive reference serum) were subjected to urea-polyacrylamide gel electrophoresis, and the gels were stained with silver. Untreated total RNAs were also applied as references of cellular RNA components. An arrow indicates 7 − 2 RNA, a component of Th/To antigen. b Immunoprecipitation-immunoblot assay for our patient’s serum. The immunoprecipitates obtained by serum samples (our patient’s serum, anti-Th/To-positive reference serum, anti-PM-Scl-positive reference serum, and healthy control serum) were subjected to immunoblots probed with rabbit anti-hPOP1 and anti-PM-Scl-100 antibodies. Immunoreactive bands were visualized by a chemiluminescence detection system. An arrow indicates 120-kDa hPOP1 and an arrowhead indicates 100-kDa PM-Scl-100

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Pediatric Rheumatology

ISSN: 1546-0096