Volume 9 Supplement 1

Proceedings of 18th Pediatric Rheumatology European Society (PReS) Congress

Open Access

Role of CD4+CD25hiCD127lo/-FoxP3+ regulatory T lymphocytes in the pathogenesis of Behçet’s disease in children

  • TA Tran1,
  • S Monteil2,
  • A Letierce3,
  • B Terrier2,
  • G Geri2,
  • D Saadoun4,
  • I Kone-Paut1,
  • B Salomon5 and
  • M Rosenzwajg2
Pediatric Rheumatology20119(Suppl 1):P84

https://doi.org/10.1186/1546-0096-9-S1-P84

Published: 14 September 2011

Introduction

Behçet’s disease (BD) is an idiopathic multisystem recurrent inflammatory disorder. Physiopathology of BD shows a role of neutrophils and cytotoxic T lymphocytes.

Our aim

Were to assess the role of regulatory T lymphocytes (Tregs) in the pathogenesis of BD in children.

Patients and methods

19 patients with active BD (group A) and 8 patients with inactive BD (group B) were compared with 25 healthy controls (group C). Percentages of blood CD4+CD127-CD25hiFoxP3+ Tregs and other T/B and NK cells subpopulations were nalayzed by flow cytometry. The frequency of IL-17A and IFN-γ producing T cells was analyzed by flow cytometer from PBMC after 4 hours stimulation with PMA-ionomycin. We measured serum cytokines by Luminex and ELISA. We compared the 3 groups by using the Wilcoxon-Rank-signed test. Values were expressed as mean and median.

Results

Patients in the 3 groups (A, B, C respectively) were comparable in term of age and sex distribution (median age: 12.8, 9,9 and 9.7; F/M = 1/1). No differences were observed between the 3 groups concerning the absolute number of lymphocytes, CD4+ T cells and the percentage of total Tregs (median: A: 1.9, B:1.1, C:2.8) . Percentages of naïve Treg/memory Treg and markers of Treg function (GITR, LAP, CD152, DR) were also similar in the 3 groups. However, there was increased CD8+ T cells count in the BD patients groups compared to healthy controls (A: 552±361, p=0.18; B: 627±159, p=0.04, C: 479±209). The NK cell (CD3-CD16+CD56+) were highest in group C compared to group A (p=0.4) or B (p=0.001). IL-17A secreting CD4+ T cells were significantly higher in active BD patients (n=6) compared to controls (n=6) (5.3±2 vs 2.5±1.47, p=0.043). Serum IL-6 level was significantly hisgher in BD populations compared to controls subjects (A: 4,3±1,22 vs C:3±0,7 pg/ml, p=0,016).

Conclusion

There is no deficit of Tregs number in BD patients. The high rate of peripheral IL-17 secreting CD4+ T cells suggests a possible role of Th17 cells in the occurrence of BD attacks. The Tregs functional ability to regulate CD4 and CD8 T cells needs to be studied further.

Authors’ Affiliations

(1)
Department of Paediatrics, Pediatric Rheumatology. CEREMAI Bicêtre Hospital, University of Paris Sud.
(2)
Service de Biothérapies/ UPMC CNRS 7211 INSERM 959, La Pitié Salpétrière University Hospital
(3)
Unité de Recherche Clinique Paris Sud, Bicêtre University Hospital
(4)
Department of Internal Medicine, La Pitié Salpétrière University Hospital
(5)
Unité 2. UPMC-CNRS U7087, La Pitié Salpétrière University Hospital

Copyright

© Tran et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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