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Volume 9 Supplement 1

Proceedings of 18th Pediatric Rheumatology European Society (PReS) Congress

Toll-like receptor 2 polymorphism in juvenile dermatomyositis

Pediatric Rheumatology volume 9, Article number: O40 (2011) Cite this article

Background

Juvenile dermatomyositis (JDM) is characterised by chronic inflammation in skeletal muscle, skin, and other target organs. Prognosis has improved in the past 10-20 years, but it is still a disease with high morbidity and considerable sequelae such as muscle weakness, joint contractures, calcinosis and lipodystrophy. There is increasing evidence that single nucleotide polymorphisms (SNPs) are associated with chronic inflammatory diseases. In the present study, we investigated known SNPs within genes that previously have been associated with inflammatory myopathies, including JDM and dermatomyositis.

Aim

The aim of the study was to investigate known polymorphisms within the genes encoding Toll-like receptor (TLR)1-10; nucleotide binding oligomerisation domain (NOD)1-2; interferon-induced helicase C domain-containing protein 1 (IFIH1); and melanoma differentiation associated gene 5 (MDA5), as well as single nucleotide polymorphisms (SNPs) in tumour necrosis factor (TNF)-alpha and protein tyrosine phosphatase, non-receptor type 22 (PTPN22) and their association with the disease course of Juvenile Dermatomyositis (JDM) in a population-based follow-up study.

Methods

In 53 JDM patients SNPs in the pattern recognition receptor genes encoding TLR1-10, DDX58 (RIG-I), IFIH1 (MDA5), NOD1 (CARD4), and NOD2 (CARD15) were analysed using an in-house multiplex bead-based assays. The TNF-alpha (rs1800629) and PTPN22 (rs2476601) polymorphisms were genotyped by pyrosequencing.

Results

The TLR2-rs1898830 SNP was found to be associated with calcinosis (p=0.006) and lipodystrophy (p=0.0006). Patients with the G/G genotype had a significantly higher risk (OR: 60.0, CI: 5.0-719.0) of developing lipodystrophy than patients with the A/A or A/G genotypes.

No other SNPs were significantly associated with JDM outcome.

Conclusion

The present study suggests an association between TLR2-rs1898830 and disease outcome in JDM.

Author information

Authors and Affiliations

  1. Dept.of Paediatrics, Holbaek University Hospital, Denmark

    P Mathiesen

  2. Institute for Inflammation Research, Dept. of Rheumatology, Rigshospitalet, University Hospital of Copenhagen, Denmark

    C Enevold & K Müller

  3. Dept. of Biostatistics, University of Copenhagen, Denmark

    T Lange

  4. Laboratory of Molecular Medicine, Dept. of Clinical Immunology, Rigshospitalet and Allogeneic Hematopoietic Cell Transplantation Laboratory, University Hospital of Copenhagen, Denmark

    B Kornblit

  5. Dept. of Hematology, Rigshospitalet,Paediatric Clinic II, Rigshospitalet, University Hospital of Copenhagen, Denmark

    S Nielsen & K Müller

Authors
  1. P Mathiesen
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  2. C Enevold
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  3. T Lange
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  4. B Kornblit
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  5. S Nielsen
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  6. K Müller
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Corresponding author

Correspondence to P Mathiesen.

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This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Mathiesen, P., Enevold, C., Lange, T. et al. Toll-like receptor 2 polymorphism in juvenile dermatomyositis. Pediatr Rheumatol 9 (Suppl 1), O40 (2011). https://doi.org/10.1186/1546-0096-9-S1-O40

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  • DOI: https://doi.org/10.1186/1546-0096-9-S1-O40

Keywords

  • Dermatomyositis
  • Protein Tyrosine Phosphatase
  • Lipodystrophy
  • Calcinosis
  • Inflammatory Myopathy

Pediatric Rheumatology

ISSN: 1546-0096