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  • Oral presentation
  • Open Access

Role of IL-1b in NLRP12-associated autoinflammatory disorders and resistance to anti-IL-1 therapy

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Pediatric Rheumatology20119 (Suppl 1) :O31

  • Published:


  • Receptor Antagonist
  • Putative Role
  • Anakinra
  • Disease Manifestation
  • Potential Interest


A new class of autoinflammatory syndromes called NLRP12-associated disorders (NLRP12AD) has been associated with mutations in NLRP12. Conflicting data on the putative role of NLRP12 in IL-1b signaling have been generated in vitro.


This prospective study was undertaken to assess the secretion of IL-1b and three IL-1b-induced cytokines (IL-1Ra, IL-6 and TNF-a) in patients’ PBMC cultured ex vivo and to evaluate the patients’ response to recombinant IL-1 receptor antagonist (IL-1Ra, anakinra), a major drug in the treatment of autoinflammatory disorders.


Patients’ disease manifestations and cytokine measurements were recorded before anakinra treatment was started, during 14 months of therapy, and after discontinuation of anakinra treatment.


Spontaneous secretion of IL-1b by patients’ PBMC was found to be dramatically increased (80 to 175-fold) compared to controls. Consistently, anakinra initially led to a marked clinical improvement and to a rapid near-normalization of IL-1b secretion. However, a progressive clinical relapse occurred secondarily, associated with an increase in TNF-a secretion, persistent elevated levels of IL-1Ra and IL-6 and a reactivation of IL-1b secretion. Anakinra was discontinued after 14 months of therapy.


Our findings provide in vivo evidence of the crucial role of IL-1b in the pathophysiology of NLRP12AD. This is the first time anakinra has been used to treat this disorder. This study provides new insights into the mechanisms underlying resistance to anti–IL-1 therapy observed in few patients with autoinflammatory syndromes. Our data also point to the potential interest of cytokine ex vivo measurements as predictors of response to treatment.

Authors’ Affiliations

INSERM, Paris, France
Centre Hospitalier de Versailles, Versailles, France
Université de Poitiers, Poitiers, France
Assistance Publique - Hôpitaux de Paris, Paris, France


© Jeru et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.