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DIssociation of T lymphocyte subpopulations in patients with juvenile idiopathic arthritis


Introduction and objective: it is well known fact that the key point in the development of an autoimmune response in rheumatoid inflammation is the dissociation between subpopulations of T lymphocytes.


The aim of our study was to analyze the quantitative changes in the spectrum of T-lymphocytes and the activity of the pathological process in children with juvenile idiopathic arthritis ( JIA ).


Materials and Methods: the main subpopulations of T - lymphocytes in peripheral blood were determined by laser flow cytometer - FacsCalibur using the program Cell-Quest. The study was conducted in patients with different stages of JIA. A following panel of antibodies: CD45/CD14, IgG1/IgG2,; CD3/CD19, CD4/CD8, CD3/HLA-DR, CD16/56, CD71 , CD95/CD54 , CD38 was used to identify lymphocyte populations.


Results of our study revealed the elevated levels of lymphocytes expressing CD3 + CD19 markers - 27,3 ± 3,4% ( compared with the reference parameters - 9.5 ± 1.1%). Besides, decreasing of CD3+19-T-lymphocytes (51,6 ± 2,4% compared to healthy 76,2 ± 1,5%), was in direct correlation with the high activity of the process ( P <0.05). Moreover, it was necessary to define two groups of results:

1 - a significant increase in T-helper cells ( CD4 + CD8-) to 44,9 ± 4,2% ( control group -34.7 ± 2.1%) while the number of CD8 + Tcytotoxic cells was within normal parameters . These results indicate the predominant contribution of 2and 3 types hypersensitivity, which are cha-racterized with the production of autoantibodies during the pathology process.

2 - preservation of T -helper population within the reference values while the content of T CD8 + effectors was increased that indicates the cell type of hypersensitivity. Growth of CD8 + T cells correlated with the activity of the process, while remaining normal in oligoarthritis with low laboratory activity (ESR, CRP). Deterioration of articular changes followed by increased levels of CD95+T-lymphocytes (12,8 ± 1,9% when a rate of healthy is 3,2 ± 0,6%). In our opinion, direct correlation between the CD95+T lymphocytes and CD8+Tcytotoxic cells indicated the dependence between proliferation, cytotoxicity and apoptosis . The level of activated CD3 + HLA-DR+ T cells was significantly increased in JIA up to 9,7 ± 1,5% ( com-pared with healthy children - 4 , 1 ± 0,5%). In one patient with systemic JIA (stage of severe rheumatoid inflammation) the level of activated CD3 + HLA-DR + T lymphocytes increased dramatically up to 38.7 %. It is necessary to point that our results did not reveal the growth of the serum immunoglobulins.


Conclusion: dissosiation of T-lymphocyte subpopulations in children with JIA correlated with clinical activity of the disease. Screening of T lymphocytes populations is promising for a personified therapy selection in patients with JIA .

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated.

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Minira, B., Aida, A. DIssociation of T lymphocyte subpopulations in patients with juvenile idiopathic arthritis. Pediatr Rheumatol 12 (Suppl 1), P210 (2014).

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