Volume 12 Supplement 1

Proceedings of the 21st European Pediatric Rheumatology (PReS) Congress

Open Access

Exploring lymphocyte subpopulations in children with behcet disease, a new diagnostic tool?

  • Stefano Ghirardelli1,
  • Antonella Meini1,
  • Marco Cattalini1,
  • Daniele Moratto2,
  • Manuela Baronio2,
  • Alessandro Plebani1 and
  • Vassilios Lougaris1
Pediatric Rheumatology201412(Suppl 1):P126

https://doi.org/10.1186/1546-0096-12-S1-P126

Published: 17 September 2014

Introduction

Behçet Disease (BD) is a chronic systemic vasculitis, with its typical onset during young adulthood. Pediatric onset of the disease may occur in a limited number of cases, and frequently the diagnostic criteria applied for adult patients may not be fully satisfied. Therefore in the last years new diagnostic criteria for pediatric-onset BD have been developed (PED-BDIC). The pathogenesis of BD is still not clear, although various immunological hypotheses have been proposed. In this regard, evaluation of peripheral lymphocyte subsets as indicators of the underlying immunological disturbance has been performed in a limited number of adult BD patients, but data in pediatric BD patients are lacking.

Objectives

T he aim of this study was to investigate the peripheral lymphocyte subsets in pediatric BD, in order to evaluate if there exists an underlying immunological disturbance.

Methods

30 children with BD followed at our Centre were included in this study. Patients were divided into 2 groups depending on the age at enrolment: <12 years of age (Group 1) and >12 years of age (Group 2); no differences in the disease duration in the two groups were noted. Absolute values of lymphocytes subpopulations from peripheral blood samples were analyzed using a flow cytometer FACSCalibur (Becton Dickinson, CA). Fluorescence data were acquired using the “Cell Quest Pro” software (Becton Dickinson, CA) and processed by “FlowJo” software (Tree Star, OR). Data were compared to age matched healthy donors (HD)

Results

The distribution of peripheral lymphocyte subsets showed several differences between BD patients and HD. In particular in Group 1, CD4+ Naïve Central Memory and Effector Memory subpopulations were decreased when compared to HDs. CD8+ Total and Effector Memory T cells resulted also decreased when compared to HDs. Regarding B cells, an increase in Switched Memory B cells was noted, when compared to HDs. In Group 2, CD4+ Naïve and Central memory T cells were decreased when compared to HDs. CD8+ Total and Naïve T cells resulted reduced as well. B cells in Group 2 did not show any significant alterations. All the differences in lymphocyte subsets described reached statistical significance.

Conclusion

We herein provide the first evidence of peripheral lymphocyte subsets disturbances in pediatric BD. These lymphocyte disturbances resulted more pronounced in younger BD patients (<12 years of age). Of interest, the majority of the data here described appear exclusive of the pediatric form of BD, since similar findings have not been reported in adult BD patients. Our findings may become useful during the diagnostic approach, especially in younger patients. Further studies in order to validate these results are ongoing.

Disclosure of interest

None declared.

Authors’ Affiliations

(1)
Pediatric Clinic, Department of Clinical and Experimental Sciences, University of Brescia
(2)
Laboratory for Molecular Medicine "A. Nocivelli", Spedali Civili di Brescia

Copyright

© Ghirardelli et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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