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PReS-FINAL-2306: Molecular analysis of HLA-DRB1 alleles in Iranian children with juvenile systemic lupus erythematosus
© Shiari et al.; licensee BioMed Central Ltd. 2013
Published: 5 December 2013
Systemic lupus erythromatosus (SLE) is a complex and systemic autoimmune disease. It is characterized by diverse clinical symptoms, revealing widespread immune-mediated damage. The common clinical features diagnosed in patients with SLE comprise of skin and joint diseases, hematological abnormalities, renal disease and neuropsychiatric complications. Although the etiology of SLE is still unknown, genetic factors are likely to be important in susceptibility to SLE and influence presentation of disease heterogeneity and production of autoantibody in affected subjects.
There are several evidences that Human leukocyte antigens are associated with SLE. Herein, we studied HLA-DRB1 alleles to detect the association of these alleles in Iranian children with juvenile onset SLE.
This study consists of 31 children with systemic lupus erythematosus and 56 healthy controls. Genomic DNA was extracted and HLA typing was performed by Polymerase Chain Reaction with Sequence-Specific Primers technique (PCR-SSP).
HLA- DRB1*01, HLA- DRB1*04, HLA-DRB1*11 and HLA- DRB1*13 were detected to be the most frequent alleles associated with SLE in Iranian children. The frequency of HLA-DRB1*08 was not significantly different in both groups. HLA-DRB1*07 had a higher rate of repetition in the control group than patients with SLE.
We reached the conclusion that there was a significant difference in the frequency of some alleles between patients and controls which could be related to susceptibility to SLE. This difference may help to predict the onset of lupus in children.
Disclosure of interest
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.