PReS-FINAL-2173: Protein kinase C delta deficiency is a new cause of monogenic SLE
Pediatric Rheumatology volume 11, Article number: O8 (2013)
Systemic lupus erythematosus (SLE) is a prototype autoimmune disease. Infectious triggers, genetic background, immunological abnormalities and environmental factors are all supposed to interact in disease development. Rare causes of monogenic SLE have been described, (e.g. complement deficiencies, interferonopathies and FasL deficiency) providing unique insights into fundamental mechanisms of immune tolerance.
Our objective was to identify the cause of an autosomal recessive form of SLE in an inbred family with three affected siblings.
We investigated three siblings and used next generation sequencing to identify mutations in the disease-associated gene. We performed extensive biochemical, immunological and functional assays to assess the impact of the identified mutations on B cell biology.
Genetic mapping and targeted exome sequencing lead to the identification of a homozygous mutation in PRKCD, encoding protein kinase C delta (PKCδ). Mutation of PRKCD resulted in reduced expression and activity of encoded protein PKCδ. In mouse, PKCδ plays a crucial role in the deletion of autoreactive B cells. As for mice deficient in PKCδ, we demonstrated that B cells display a resistance to calcium-dependent apoptosis and a higher proliferation rate associated with an increase of immature B cells in affected patients, and a developmental shift toward an immature phenotype of naïve B cells.
Our findings indicate that PKCδ is crucial in regulating B cell tolerance and preventing self-reactivity in humans.
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Belot, A., Kasher, P., Trotter, E. et al. PReS-FINAL-2173: Protein kinase C delta deficiency is a new cause of monogenic SLE. Pediatr Rheumatol 11 (Suppl 2), O8 (2013). https://doi.org/10.1186/1546-0096-11-S2-O8