- Meeting abstract
- Open Access
OR10-002 - A novel TNFR1 transcript of TRAPS gene
Pediatric Rheumatologyvolume 11, Article number: A186 (2013)
Mutations in the TNFRSF1A gene encoding the TNF cell surface receptor, TNFR1, cause TNFR-associated periodic syndrome (TRAPS) and polymorphisms in TNFRSF1A, including rs4149570, rs767455 and rs1800692, are associated with inflammatory diseases.
We describe a novel exon 2-spliced transcript, named TNFR1-d2, and the impact of these 3 SNPs on exon 2 splicing, transcriptional activity of TNFRSF1A and TRAPS phenotype.
Expression of TNFRSF1A transcripts was performed by RT-PCR in a range of human cells and tissues. Exon 2 splicing and transcriptional activity were analysed in HEK293T and SW480 cells by in vitro alternative splicing and luciferase assays, respectively. We constructed haplotypes containing rs4149570, rs767455 and rs1800692 in controls (n=70), TRAPS (n=111) and TRAPS-like patients (n=450) to compare their distribution and association with clinical features of TRAPS.
TNFR1-d2 was expressed in a tissue-specific manner, whereas TNFR1 expression was ubiquitous. Alternative splicing assays revealed that the T-A-T haplotype at rs4149570-rs767455-rs1800692 showed the highest expression of exon 2-skipping product (p=0.02). Transcriptional activity from the T-T haplotype at rs4149570-rs1800692 was increased compared to the G-C haplotype (p=0.03). In TRAPS patients, rs1800692 T/T homozygotes were excessively rare (p<10-4) and TRAPS-like patients with this genotype experienced less fever.
Our study provides a novel mechanism of TNFRSF1A regulation whereby three polymorphisms in the promoter, exon 1 and intron 4 have a functional and combined effect on exon 2 splicing, via a coupling mechanism between transcription and splicing. These polymorphisms may impact the phenotype of TRAPS and TRAPS-like patients.