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Table 1 PCR and pyrosequencing primers used in the analysis of promoter variants in DC-SIGN

From: DC-SIGN gene promoter variants and IVIG treatment response in Kawasaki disease

SNP

Variants†

First round nested PCR

Pyrosequencing primer

rs#

(5’-3’)

(Nucleotide position)*

  

rs2287886

G/a

F: Biotin AGCTTTTATTTCCCACCCTGTGATC

TAGY CTTTTGGATGACAGAT CCCTACCCA

(-139)

R: CTTGAAAGATCCGGCCCATCTC

S: GCTCTGATGCTTTCCAC

rs4804803

A/g

F ATGGTCTGGGGTTGACAGGGAG

GGTR GGCAGGTAGCACCCCC AGTTCCTGG

(-336)

R Biotin GAGGACAGCAGCAGCTCAAAGC

S CCTCCACTAGGGCAAG

rs735240

G/a

F Biotin CAGTAAAAGGACCCAGACAGAGTGAG

ACAY AAAATCAAATCTTACA GTGTGTACT

(-939)

R CCTTCAAAGGTTAGTTCTGCCTGTTC

S GCCTGTTCTCAAAATTC

rs11465360

C/a

F Biotin CAAAGTAAAGATCAGGTCGAAAACCAC

ATGTK TGTTAAATCTCAGTT TAAAGATGGA

(-1089)

R GGGGTTTCTGTATGCTGGAAATG

S GATGTGCATTTACCAC

rs4804804

G/a

F AGAAACCAGCAGGAACCAGCAG

TR GGCATAAGATACTCCCTC CAGTGCC

(-1466)

R Biotin GTCATGGCACTGGAGGGAGTATC

S CTGCCTCATTGCTCG

  1. *Nucleotide position as described in Martin et al. [14]; † second variant in small letters is the minor allele as described in HapMap Whites.
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Pediatric Rheumatology

ISSN: 1546-0096