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Fig. 2 | Pediatric Rheumatology

Fig. 2

From: Methotrexate inhibits BMP4 and abrogates the hypertrophic chondrocyte phenotype of synovial fibroblasts in juvenile idiopathic arthritis

Fig. 2

Chondrocyte marker expression in persistent, ETB, and polyarticular samples and methotrexate treatment on FLS from persistent, ETB, and polyarticular. Col2 was significantly increased in polyarticular JIA FLS when compared to ETB JIA FLS (FC = 1.02; p = 0.032) (A). ColX was significantly increased in polyarticular JIA FLS when compared to both persistent JIA FLS (FC = 1.50; p = 0.031) and ETB JIA FLS (FC = 1.37; p = 0.042) (A). In persistent JIA FLS, BMP4 protein expression decreased by − 1.20-fold in treated cells compared to untreated cells (p = 0.018) (B). BMP4 decreased by − 1.20-fold in ETB JIA FLS treated cells compared to untreated cells (p = 0.020) (B). Lastly, in the polyarticular JIA FLS, cells treated with methotrexate had a − 1.24-fold decrease in total BMP4 protein compared to untreated cells (p = 0.010) (B)

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Pediatric Rheumatology

ISSN: 1546-0096