Prevalence and Significance of Serum 14-3-3η(eta) in Juvenile Idiopathic Arthritis

Background Prompt diagnosis of juvenile idiopathic arthritis (JIA) is important to avoid long term complications. Elevated serum 14-3-3η levels improve the diagnostic sensitivity of rheumatoid factor (RF) and cyclic citrullinated peptide (CCP) antibody in adult rheumatoid arthritis (RA), and have been associated with more severe phenotype. We investigated the prevalence and clinical significance of serum 14-3-3η in different types of JIA. Methods JIA patients (n = 152) followed by the Pediatric Rheumatology Core at Children’s Hospital of Los Angeles were categorized into 5 groups: polyarticular JIA RF+ (PJIA RF+; n = 39), PJIA RF- (n = 39), psoriatic arthritis (PsA; n = 20), enthesitis-related arthritis (ERA; n = 18), and oligoarticular JIA (OJIA [control group]; n = 36). RF, CCP antibody, and 14-3-3η were measured for all patients. 14-3-3η serum levels ≥ 0.2 ng/mL were considered positive. Disease activity was assessed by the Juvenile Arthritis Disease Activity Score-71 (JADAS-71). Results Elevated 14-3-3η levels were detected in 35/152 (23%) patients, and across all groups tested. Most patients with 14-3-3η had titers ≥ 4 times above the cutoff value. The majority (23, 67%) of 14-3-3η-positive patients were also positive for RF or CCP antibodies, 17 (49%) were positive for all 3, and 12 (8%) were single-positive for 14-3-3η. The highest prevalence of 14-3-3η was in PJIA RF + patients (49%), followed by OJIA (22%). Positivity for 14-3-3η was not significantly associated with disease activity or age at diagnosis. Conclusion Serum 14-3-3η can be detected in all forms of JIA tested but appears to be most common in PJIA RF+. 14-3-3η does not appear to correlate with disease activity in JIA.


Background
Juvenile idiopathic arthritis (JIA) is the most common pediatric rheumatologic disease that may persist into adulthood, leading to alterations in quality of life. The diagnosis is mainly based on clinical assessment rather than relying on laboratory testing. The disease course and prognosis of JIA may vary based on multiple factors such as presence of the biomarkers rheumatoid factor (RF) and cyclic citrullinated peptide (CCP) antibodies [1, 2,3]. RF-seropositive polyarticular JIA (PJIA RF+) has similar features as rheumatoid arthritis (RA) and may extend into adulthood. Thus, PJIA RF + patients are more susceptible to developing severe disease and are at higher risk of erosive joint damage [1, 4,5]. Based on a 17-year cohort study, outcomes are best predicted at 5-year follow up, since there are no specific diagnostic markers for JIA [6].
The biomarker 14-3-3η is highly sensitive and specific for RA in adults. 14-3-3η is a protein that activates proinflammatory cytokines in patients with RA [7,8]. There are 7 isoforms of the 14-3-3 protein. Only 14-3-3η is found in the serum and synovial fluid in patients with RA, but at a 3-5 fold higher concentration in synovial fluid compared to matched serum, consistent with intraarticular production [8,9]. Elevated serum 14-3-3η levels improve the diagnostic sensitivity of RF and CCP antibody in adult RA and are associated with a more severe RA phenotype [8]. The combination of the 3 serum markers (presence of 14-3-3η, RF, and/or CCP antibodies) has been reported to increase the diagnostic sensitivity to 78% for early RA and 96% for established RA [7,8]. Based on previous studies serum 14-3-3η has enhanced the detection of early-RA by 32% and 22% over RF and CCP antibody, respectively [8]. Serum 14-3-3η titer may also have prognostic utility. A few studies have reported an association of elevated 14-3-3η with development of RA in patients with arthralgia [10,11,12]. In addition, patients with early RA and detectable 14-3-3η at baseline had more erosive disease at 5 years compared to those with normal 14-3-3η levels at baseline [11]. Further, patients with adult RA in apparent clinical remission after 18 months of therapy had worse Sharp scores at 30 months if their 18-month 14-3-3η level was ≥ 0.5 ng/mL [13]. Additionally, psoriatic arthritis patients have been reported to have more erosive disease at a lower level of 14-3-3η compared to RA patients [8].
The prevalence and sensitivity of this biomarker have not been studied in a large pediatric population with JIA, except for three abstract publications of much smaller pediatric populations [14,15,16]. This study reports the prevalence and clinical significance of serum 14-3-3η in a larger population of children with various subtypes of JIA.

Materials And Methods
Patient Cohort: Patients for this study were recruited from the patient population examined and followed by the Pediatric Rheumatology Core at Children's Hospital of Los Angeles (PRC-CHLA). Patients with a diagnosis of OJIA, PJIA RF+, PJIA RF-, ERA, and PsA were enrolled. Subjects met the diagnostic International League of Associations for Rheumatology (ILAR) criteria of OJIA, PJIA RF+, PJIA RF-, and ERA. Subjects were diagnosed with psoriatic arthritis based on Vancouver criteria, as there was a patient with psoriatic arthritis with a positive RF who would not qualify for ILAR diagnostic criteria. Children with a history of other autoimmune disorders or other forms of arthritis were excluded from the study. The protocol was reviewed and approved by the Children's Hospital of Los Angeles institutional review board (IRB: CHLA-15-00482). Consent and assent were obtained from all subjects in order to participate in the study.
A one-time blood draw (1 red top tube ~ 5 mL) for 14-3-3η via venipuncture was obtained during a routine laboratory and rheumatology visit, or scheduled infusion, from 152 patients: 39 with PJIA RF+, 39 with PJIA RF-, 36 with OJIA, 18 with ERA, and 20 with PsA. Other laboratory studies, including erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP), were obtained at the same time. Specimens for CCP antibody, RF, antinuclear antibody (ANA), and HLA-B27 testing were obtained at the same time as well, if the values were missing in their chart (paper and/or electronic).

Analyses of serum 14-3-3η
Samples for 14-3-3η were centrifuged and the serum was collected. Serum was stored in a freezer at CHLA. Then the batch was packaged on dry ice and mailed to Quest Diagnostics overnight. 14-3-3η was measured via ELISA at Quest Diagnostics [2]. A 14-3-3η serum level of ≥ 0.2 ng/mL was considered positive. Fisher's exact test was used to assess differences in prevalence of positive 14-3-3η between the OJIA (control) group and all other groups. The Cochran-Armitage trend test was used to evaluate the linear association of positive 14-3-3η with composite RF and CCP antibody positivity. Fisher's exact test was also used to assess the association of 14-3-3η positivity with ANA and CRP.

Serum 14-3-3η vs. age of onset and age at the time of blood draw
Differences in age at time of diagnosis as well as age at time of blood draw for 14-3-3η positive and negative subjects were assessed with Kruskal-Wallis tests. All other comorbidities and medications were obtained from the patient chart.
Comparison of 14-3-3η, RF, and CCP antibodies within each group: OJIA subjects had the highest rate of positive 14-3-3η (19%) in the absence of other biomarkers: RF or CCP antibody ( Table 2). Of note, 8 out of 36 (22%) of the OJIA patients had detectable 14-3-3η, most of whom (88%) were positive for only 14-3-3η (negative for RF and CCP antibodies; Table 2). The OJIA and ERA groups were the least likely to be positive for 14-3-3η, in addition to RF or CCP antibody or both. Overall, the PJIA RF + group had a higher proportion of triple positive patients with 14-3-3η, CCP antibody, and RF (41%) than any other group.
# Note the calculated p-value for all 3 biomarkers being positive across all groups is 1.3 × 10 7 with a Z score = -5.3.
Positive CCP antibody levels were more commonly observed in PJIA groups. However, OJIA, PsA, and ERA groups were more likely to have a positive 14-3-3η compared to CCP antibody. Twenty two percent of OJIA subjects had a detectable 14-3-3η level, while only 6% had a positive CCP antibody. However, PJIA RF + group had a higher prevalence of CCP antibody (77%) vs. 14-3-3η (49%).

Comparison of 14-3-3η and disease activity:
There was no association between disease activity based on JADAS-71 and positive 14-3-3η results, in any of the JIA types (Table 3). There was no association between age of onset, age at the time of blood draw, and having a positive 14-3-3η titer. Furthermore, no correlations between detectable 14-3-3η level and treatments (NSAIDs, DMARD, or biologics) were observed (Table 4). Methotrexate and anti-TNFα were the most common therapies used. Interestingly, a large proportion of OJIA patients required DMARD (81%) and/or anti-TNFα (42%) therapy. OJIA patients required biologics less commonly compared to the other groups. All of the OJIA subjects with positive 14-3-3η titer required DMARDs, 38% of whom also required biologics. However, all of PJIA RF-subjects with elevated 14-3-3η level required biologics, which was different than PJIA RF+ (84%), PsA (67%), and ERA (50%). Table 3 JIA Disease Activity vs. 14-3-3η

Discussion
To date this is the largest pediatric study published assessing the importance of 14-3-3η in children with JIA. Overall in our study, the prevalence of JIA was much higher among female patients (78%), compared to previous reports of a 2 to 1 female to male ratio, respectively [1, 5,17]. PJIA RF + mimics adult RA and, as expected, the PJIA RF + population had the highest prevalence of being positive for 14-3-3η (49%). There was a positive correlation with positive 14-3-3η among PJIA RF + group when compared to OJIA (p value = 0.03) and PsA (p value = 0.012). The prevalence of 14-3-3η positivity in children with PJIA RF + was similar to that in another study on early undifferentiated polyarthritis in adults. However, in both early and established RA, a higher prevalence of 14-3-3η (68%) was reported compared to our pediatric population [17,18]. In a previous report, 14-3-3η was positive in 21% of patients with early RA who were seronegative for RF and CCP antibodies, and in 67% of patients with seronegative established RA and in a pediatric study, 31% of PJIA RF-patients had positive 14-3-3η levels [15,19]. This differs from our results, which showed a much lower frequency (8%) in the PJIA RF-group.
To date there are only two pediatric 14-3-3η studies on JIA, which were published as conference abstracts. These studies had a smaller population of JIA patients. Similar to our study, the highest prevalence of positive 14-3-3η level was seen among PJIA RF + patients [14,15,16]. There were some differences between these reports: Rosenberg et al study described no significant association between positive RF and 14-3-3η titer in JIA, yet a correlation between positive 14-3-3η and positive RF and CCP antibodies in PJIA was observed in Feller/Dalrymple et al study [14,15,16]. We found a positive correlation between 14-3-3η biomarker, RF, and CCP antibodies (p-value = 1.3 × 10 7 ) in all groups. Serum 14-3-3η was found in all groups, including ERA subjects (11%). This finding differs from the Rosenberg et al study, which reported no detectable 14-3-3η among their 4 ERA patients [16]. Dalrymple et al group described positive serum 14-3-3η among all JIA groups (PJIA RF+, PJIA RF-, systemic JIA, OJIA), RA, as well as systemic lupus erythematosus and healthy control groups [15]. The age group of these patients was not provided [15].
CCP antibody was more commonly observed in the PJIA RF + group, similar to prior reports [20,21,22]. In our study, all groups except the ERA population had a positive CCP antibody, yet at a much lower frequency when compared to the PJIA RF + group. There is a large variance in the reported prevalence of CCP antibody among different studies, with a positive association between RF and CCP antibodies [23,24].
As expected, positive ANA serology was most commonly seen among the OJIA population [4]. Overall, there was a high prevalence of positive ANA among all groups, but to a lesser degree in PJIA RF-patients. However, in the presence of positive 14-3-3η titer, PJIA RF + patients had the highest prevalence of positive ANA. No PJIA RFpatients had a positive 14-3-3 positive in the presence of a positive ANA result. Based on our search, there are no studies comparing the prevalence of positive ANA and 14-3-3η. Our study did not find any association between positive ANA and 14-3-3η.
In RA, 14-3-3η analysis has enhanced the detection of early-RA over RF and CCP antibodies, and it is associated with worse disease [8,11]. Serum 14-3-3η was found to be a predictive biomarker to determine evolving RA and radiographic changes in early RA [10,11]. Even though elevated 14-3-3η was reported to be associated with radiographic damage and disease progression in adults with established RA, no significant association between positive 14-3-3η and Disease Activity Score -28 (DAS28) was reported [9,19]. In our cohort, we also did not note any association between positive 14-3-3η and JADAS-71 in children with JIA [9]. A prior adult study, in abstract form, reported that 14-3-3η levels were modifiable after treatment with adalimumab and were predictive of treatment response in adults with PsA and RA [25]. Nevertheless, another article revealed no difference in pretreatment and posttreatment 14-3-3η levels in in RA patients treated with adalimumab, tofacitinib, or methotrexate [26]. Conversely, tocilizumab pretreatment 14-3-3η levels were predictive of remission and corresponded to DAS28 after therapy [26]. We found no correlation between positive 14-3-3η titer and administered therapy: NSAIDs vs. DMARDs vs. biologics. Many patients had elevated 14-3-3η titers even with exposure to biologics. Both OJIA and PJIA RF-patients with positive 14-3-3η were treated with DMARDs and biologics, respectively. Still, a high proportion (89%) of PJIA RF + group with positive 14-3-3η required biologics.
OJIA patients were the only group with positive 14-3-3η and chronic uveitis. There were no cases of chronic uveitis in the PJIA RF + group. Furthermore, no correlations between 14-3-3η titer and disease activity, age of onset or age of blood draw, were observed. Chronic uveitis was most commonly observed among OJIA and ERA patients.

Conclusion
Serum 14-3-3η titer was found in all subgroups of JIA. As expected when comparing to previous studies among RA patients and other pediatric JIA studies, PJIA RF + group had the highest prevalence of positive 14-3-3η compared to other groups. Therefore, 14-3-3η may be used as a biomarker in JIA, especially PJIARF + patients; however it is significance as a diagnostic biomarker or prognostic biomarker is still unknown. In the absence of RF and CCP antibodies, OJIA group had the highest prevalence of positive 14-3-3η and all of OJIA and PJIA RFpatients positive for 14-3-3η required therapy with DMARDs and biologics, respectively. Thus 14-3-3η may possibly be used as a measure of poor drug response and escalation of therapy among OJIA and PJIA RF-patients with positive 14-3-3η.

Limitations
Patients with new onset of JIA and established diagnosis were not differentiated. OJIA patients were used as a control group, yet there was a high prevalence of positive 14-3-3η among this group. Neither healthy subjects nor healthy children with arthralgia without evidence of arthritis were included in this study.

Future Studies
A prospective study of patients at onset of disease and follow up would be beneficial in determining the role of 14-3-3η biomarker. Comparing 14-3-3η level among JIA vs. patients with arthralgia, and healthy patients with follow up over several years would be informative in the role of this biomarker in disease classification. Additionally, measurement of 14-3-3η pretreatment and posttreatment, as well as comparison via imaging may be helpful to assess the value of 14-3-3η as a marker of joint damage and treatment response.