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Fig. 1 | Pediatric Rheumatology

Fig. 1

From: Mitigated suppressive function of regulatory T cells (Treg) upon Th17-inducing cytokines in oligo- and polyarticular Juvenile Idiopathic Arthritis (JIA) patients

Fig. 1

Influence of different stimuli on phenotype of isolated regulatory T cells. The influence of different stimuli on isolated Treg was assessed by flowcytometry after 5 days of culture and values of FoxP3+CD4+ cells in isolated regulatory T cells (Treg) are shown in percentage (median and interquartile ranges). As stimuli were used: unstimulated = none; anti-CD3/CD28 as positive control; Th17 = anti-CD3/CD28 + IL-1β + IL-6 + IL-23 + TGFβ; anti-IL17A = anti-CD3/CD28 + laboratory IL-17A-antibody; Secukinumab = anti-CD3/28 + Secukinumab. N = 6 for healthy controls (HC) and Juvenile Idiopathic Arthritis (JIA) each, except for Secukinumab in HC (here n = 5). Groups as well as stimuli were compared for differences with Mann–Whitney-U-test. Whilst elevated level of FoxP3+CD4+ cells could be seen after Th17-inducing stimuli in HC and JIA and furthermore in every stimulus for JIA compared to HC (with smallest difference in Secukinumab, p > 0.2), correction for multiple comparison with Bonferroni correction showed significant difference for FoxP3+CD4+ cells between JIA and HC after anti-IL-17A cultivation (p = 0.004, marked with *)

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