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Fig. 8 | Pediatric Rheumatology

Fig. 8

From: The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes

Fig. 8

Using ELISA, total protein expression was determined for Ch markers on FLS cultured in Ch-conditioned media and then treated with TGFBR1 inhibitor. Protein expression of COL2, a marker of proliferating Ch, COLX, a marker of Ch hypertrophy, ALP, a marker of endochondral ossification, and COMP, a marker of late Ch hypertrophy as determined by ELISA on cell media supernatants. CFLS-Ch treated with the TGFBR1 inhibitor had increased expression of ALP (p = 0.029) compared with CFLS-Ch, while COL2, COLX, and COMP remained unchanged (a). JFLS-Ch treated with the TGFBR1 inhibitor had signifcant increases in COL2 (p = 0.022) compared with JFLS-Ch, while COLX, ALP, and COMP had no significant changes (b)

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Pediatric Rheumatology

ISSN: 1546-0096