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Fig. 7 | Pediatric Rheumatology

Fig. 7

From: The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes

Fig. 7

Using antibody arrays spotted for phosphorylated proteins specific to TGFβ/BMP signaling, we measured the total intensity of phosphorylation at 24 h on cell culture supernatants from FLS cultured in conditioned media from Ch and FLS-Ch treated with a TGFBR1 inhibitor. The phosphorylation of ATF2, c-fos, c-Jun, SMAD1, SMAD2, SMAD4, SMAD5, and TAK1 remained unchanged in CFLS-Ch treated with the TGFBR1 inhibitor when compared with CFLS-Ch (a). JFLS-Ch treated with the TGFBR1 inhibitor had decreased phosphorylation of c-fos (p = 0.041) compared with JFLS-Ch (b)

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Pediatric Rheumatology

ISSN: 1546-0096