Skip to main content
Fig. 6 | Pediatric Rheumatology

Fig. 6

From: The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes

Fig. 6

Using ELISA, total protein expression was determined for Ch markers on FLS cultured in Ch-conditioned media and then exposed to exogenous BMP4. Protein expression of COL2, a marker of proliferating Ch, COLX, a marker of Ch hypertrophy, ALP, a marker of endochondral ossification, and COMP, a marker of late Ch hypertrophy as determined by ELISA on cell media supernatants. CFLS-Ch treated with exogenous BMP4 exhibited significant decreases in COL2 (p = 0.022) compared with CFLS-Ch (a). JFLS-Ch treated with exogenous BMP4 had signifcant increases in COL2 (p = 0.004) and COLX (p = 0.021) compared with JFLS-Ch, while ALP and COMP remained unchanged (b)

Back to article page

Pediatric Rheumatology

ISSN: 1546-0096