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Fig. 5 | Pediatric Rheumatology

Fig. 5

From: The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes

Fig. 5

Using antibody arrays spotted for phosphorylated proteins specific to TGFβ/BMP signaling, we measured the total intensity of phosphorylation at 24 h on cell culture supernatants from FLS cultured in conditioned media from Ch (FLS-Ch) and FLS-Ch exposed to exogenous BMP4. CFLS-Ch treated with exogenous BMP4 downregulate the phosphorylation of SMAD2 (p = 0.043), SMAD5 (p = 0.037), and TAK1 (p = 0.040) compared with CFLS-Ch (a). JFLS-Ch treated with exogenous BMP4 have increased phosphorylation of ATF2 p = 0.000, c-Fos p = 0.001, c-Jun p = 0.004, SMAD1 p = 0.009, SMAD2 p = 0.000, SMAD4 p = 0.000, SMAD5 p = 0.001, and TAK1 p = 0.000 compared with JFLS-Ch (b)

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Pediatric Rheumatology

ISSN: 1546-0096