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Fig. 3 | Pediatric Rheumatology

Fig. 3

From: The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytes

Fig. 3

Using ELISA, protein expression was determined for Ch markers. Protein expression of COL2, a marker of proliferating Ch, COLX, a marker of Ch hypertrophy, ALP, a marker of endochondral ossification, and COMP, a marker of late Ch hypertrophy as determined by ELISA on cell media supernatants. The total protein was calculated at 24 h. CFLS-Ch had significant decreases in total protein expression of COL2 (p = 0.000) and COLX (p = 0.000) when compared with CFLS (a). COMP (p = 0.009) significantly increases in CFLS-Ch compared to untreated CFLS (a). JFLS-Ch significantly downregulate expression of COL2 (p = 0.000) and COLX (p = 0.000), while ALP (p = 0.000) and COMP (p = 0.000) expression increases compared with JFLS (b)

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