Fig. 3From: The culture microenvironment of juvenile idiopathic arthritis synovial fibroblasts is favorable for endochondral bone formation through BMP4 and repressed by chondrocytesUsing ELISA, protein expression was determined for Ch markers. Protein expression of COL2, a marker of proliferating Ch, COLX, a marker of Ch hypertrophy, ALP, a marker of endochondral ossification, and COMP, a marker of late Ch hypertrophy as determined by ELISA on cell media supernatants. The total protein was calculated at 24 h. CFLS-Ch had significant decreases in total protein expression of COL2 (p = 0.000) and COLX (p = 0.000) when compared with CFLS (a). COMP (p = 0.009) significantly increases in CFLS-Ch compared to untreated CFLS (a). JFLS-Ch significantly downregulate expression of COL2 (p = 0.000) and COLX (p = 0.000), while ALP (p = 0.000) and COMP (p = 0.000) expression increases compared with JFLS (b)Back to article page