Single nucleotide polymorphisms associated with methotrexate-induced nausea in juvenile idiopathic arthritis

Table 2 Genotypes for the selected single nucleotide polymorphisms

Gene	SNP	Ancestral Allel	Genotypes	Total, n	MTX _intolerant¹	MTX tolerant	p-value	MTX Nausea²	Controls	p-value
*ABCC2*	rs2273697	G	GG	74	44	29	χ² = 0.05	32	14	0.81
			GA	32	20	12	p = 0.98	16	5
			AA	13	8	5		8	2
*ABCC2*	rs3740066³	G	GG	38	23	15	0.74	19	7	1.00
			GA	65	38	27		29	12
			AA	15	10	4		7	2
*ABCC2*	rs717620	G	GG	73	47	26	0.64	35	13	1.00
			GA	39	21	17		18	7
			AA	7	4	3		3	1
*ABCB1*	rs2032582	G	GG	38	23	15	0.28	18	6	0.95
			GT	58	34	24		27	10
			GA	2	0	2		1	1
			TA	3	3	0		2	1
			TT	18	12	5		8	3
*ABCB1*	rs1045642	C	CC	18	9	9	χ² = 1.34	8	4	0.82
			CT	68	44	24	p = 0.51	34	11
			TT	33	19	13		14	6
*SLCO1B1*	rs4149056	T	TT	84	49	34	0.86	39	15	1.00
			TC	32	21	11		16	6
			CC	3	2	1		1	0
*SLCO1B1*	rs4149081	G	GG	80	47	32	0.86	38	14	1.00
			GA	36	23	13		17	7
			AA	3	2	1		1	0
*SLCO1B3*	rs2117032	C	CC	17	12	5	χ² = 2.16	10	4	0.63
			CT	53	28	24	p = 0.34	23	6
			TT	49	32	17		23	11
*SLC19A1*	rs1051266³	G	GG	33	18	15	χ² = 0.76	17	6	0.89
			GA	66	42	24	p = 0.68	28	12
			AA	19	11	7		10	3
*HTR3A*	rs1062613	C	CC	80	52	28	0.13	36	16	0.58
			CT	37	20	16		19	5
			TT	2	0	2		1	0
*HTR3A*	rs1985242	A	AA	7	3	4	0.23	4	1	0.34
			AT	55	30	24		28	7
			TT	57	39	18		24	13
*HTR3A*	rs1176713	C	CC	6	3	3	0.68	3	1	0.18
			CT	37	21	16		19	3
			TT	76	48	27		34	17
*HTR3B*	rs1776744	T	TT	50	30	20	0.09	24	10	0.58
			TG	58	32	25		27	8
			GG	11	10	1		5	3
*MTHFR*	rs1801131³	A	AA	52	34	18	χ² = 0.93	24	9	0.29
			AC	53	30	22	p = 0.63	21	11
			CC	13	7	6		10	1
*MTHFR*	rs1801133	C	CC	58	33	25	0.02	27	10	1.00
			CT	51	29	21		24	10
			TT	10	10	0		5	1

The genotype distribution for the selected single nucleotide polymorphisms (SNPs) – including the distribution of genotypes within the phenotype subgroups defined by the methotrexate intolerance severity score and the nausea diary
SNP; Single Nucleotide Polymorphism
¹The MTX_intolerant subgroup: children with a total score of the Methotrexate Intolerance Severity Score (MISS) ≥6 and at least 1 point in the anticipatory and/or associative and/or behavioral symptoms (n = 72). Otherwise categorised as MTX_tolerant (n = 46)
²The “MTX-nausea” subgroup: children with a nausea diary illustrating a nausea pattern timely related to the MTX administration (n = 56). The remaining children grouped as “Controls” (n = 21)
³SNP variant not available for one child

ISSN: 1546-0096