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Fig. 6 | Pediatric Rheumatology

Fig. 6

From: Juvenile idiopathic arthritis fibroblast-like synoviocytes influence chondrocytes to alter BMP antagonist expression demonstrating an interaction between the two prominent cell types involved in endochondral bone formation

Fig. 6

Unsupervised hierarchal clustering comparing chondrocytes cultured in FLS-conditioned media and untreated chondrocytes and differentially expressed genes specific to TGFβ and BMP signaling in these chondrocytes. LIMMA revealed 11 differentially expressed genes with a 5% FDR when comparing Ch and Ch-CFLS (Fig. 4a, teal bar) and 13 differentially expressed genes between Ch and Ch-JFLS (Fig. 4a, purple bar) after 6 h of exposure to conditioned media. Overlapping genes revealed those regulated by TGFβ/BMP (ID1, ID3, and PMEPA1). Analyzed changes in gene expression between untreated Ch compared to Ch-CFLS and Ch-JFLS over 24 h using the curated list of genes involved in TGFβ/BMP signaling (b). Ch-CFLS and Ch-JFLS had significantly lower expression levels of TGFβ-related genes when compared to untreated Ch (Ch-CFLS: SMAD4, SMURF2, TGFBR2, TGFBI and Ch-JFLS: SMAD2, SMAD7, TGFBR2) (p < 0.05). Ch-JFLS had significantly increased expression of BMP2 and BMP7 compared to untreated Ch (p < 0.05)

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