Skip to main content
Fig. 1 | Pediatric Rheumatology

Fig. 1

From: Juvenile idiopathic arthritis fibroblast-like synoviocytes influence chondrocytes to alter BMP antagonist expression demonstrating an interaction between the two prominent cell types involved in endochondral bone formation

Fig. 1

Unsupervised hierarchal clustering comparing chondrocytes (Ch) and fibroblast-like synoviocytes (FLS) after 6 h in culture and differentially expressed genes specific to TGFβ and BMP signaling in these cells. a, Unsupervised hierarchical clustering of 104 differentially expressed genes (estimated percentage false prediction (pfp < 0.01, Rank Product) JFLS have similar gene expression patterns to CFLS and differ from Ch. b, Using a manually curated list of 27 genes specific to these signaling pathways, we examine changes in gene expression over 24 h using linear expression values. CFLS have significantly higher levels of BMP signaling via the activation of SMAD1/5 while JFLS overexpress BMP ligand, BMP7 compared to Ch (p < 0.05, t-test). Concurrently, there is a reduction in the gene expression of TGFβ receptors in CFLS and JFLS (TGFBR2/TGFBR3 and TGFBR1/TGFBR3, respectively) when compared to Ch (p < 0.05)

Back to article page

Pediatric Rheumatology

ISSN: 1546-0096