Protein analysis of ADAM17 enzyme in JSLE, JIA and control monocytes. Levels of ADAM17 were measured by flow cytometry in JSLE (n = 6), control (n = 6) and JIA (n = 6) CD14 Cells (A). A representative histogram plot of the results is shown (A). CD14 cell lysates were isolated from JSLE (n = 6), JIA (n = 7) and control (n = 7) patients and measured for ADAM17 activity (B). The presence of ADAM17 causes cleavage of a substrate which will fluoresce. The level of fluorescence is measured in fluorescent units (FU).