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Table 3 Restriction enzymes and length of the restriction fragments

From: Surface expression and genotypes of Toll-like receptors 2 and 4 in patients with juvenile idiopathic arthritis and systemic lupus erythematosus

Gene and polymorphism Restriction enzyme Restriction temperature [°C] Fragment length [bp]
TLR2 Arg677Trp MwoI 60°C Wild type (allele C): 130 bp + 22 bp
(rs unknown) Arg677Trp (allele T): 152 bp
TLR2 Arg753Gln MspI 37°C Wild type (allele G): 104 bp + 25 bp
(rs5743708) Arg753Gln (allele A): 129 bp
TLR4 Asp299Gly NcoI 37°C Wild type (allele A): 188 bp
(rs4986790) Asp299Gly (allele G): 168 bp + 20 bp
TLR4 Thr399Ile HinfI 37°C Wild type (allele C): 124 bp
(rs4986791) Thr399Ile (allele T): 98 bp + 26 bp