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Table 3 Restriction enzymes and length of the restriction fragments

From: Surface expression and genotypes of Toll-like receptors 2 and 4 in patients with juvenile idiopathic arthritis and systemic lupus erythematosus

Gene and polymorphism

Restriction enzyme

Restriction temperature [°C]

Fragment length [bp]

TLR2 Arg677Trp

MwoI

60°C

Wild type (allele C): 130 bp + 22 bp

(rs unknown)

Arg677Trp (allele T): 152 bp

TLR2 Arg753Gln

MspI

37°C

Wild type (allele G): 104 bp + 25 bp

(rs5743708)

Arg753Gln (allele A): 129 bp

TLR4 Asp299Gly

NcoI

37°C

Wild type (allele A): 188 bp

(rs4986790)

Asp299Gly (allele G): 168 bp + 20 bp

TLR4 Thr399Ile

HinfI

37°C

Wild type (allele C): 124 bp

(rs4986791)

Thr399Ile (allele T): 98 bp + 26 bp

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Pediatric Rheumatology

ISSN: 1546-0096