Surface expression and genotypes of Toll-like receptors 2 and 4 in patients with juvenile idiopathic arthritis and systemic lupus erythematosus

Table 2 Sequences of primers used for PCR

Gene and polymorphism	Primer 5′-3′	Annealing temperature [°C]
TLR2 Arg677Trp	Forward: ccc ctt caa gtt gtg g ct tca taa g	65
TLR2 Arg677Trp	Reverse: agt cca gtt cat act tgc acc ac	65
TLR2 Arg753Gln	Forward: cat tcc cca gcg ctt ctg caa gct c c	65
TLR2 Arg753Gln	Reverse:: gga acc tag gac ttt atc gca gct c	65
TLR4 Asp299Gly	Forward: agc ata ctt aga cta cta c ct cca tg	62
TLR4 Asp299Gly	Reverse: aga aga ttt gag ttt caa tgt ggg	62
TLR4 Thr399Ile	Forward: ggt tgc tgt tct caa agt gat ttt ggg a g a a	60
TLR4 Thr399Ile	Reverse: gga aat cca gat gtt cta gtt gtt cta agc c	60

ISSN: 1546-0096