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Table 2 Sequences of primers used for PCR

From: Surface expression and genotypes of Toll-like receptors 2 and 4 in patients with juvenile idiopathic arthritis and systemic lupus erythematosus

Gene and polymorphism Primer 5′-3′ Annealing temperature [°C]
TLR2 Arg677Trp Forward: ccc ctt caa gtt gtg g ct tca taa g 65
TLR2 Arg677Trp Reverse: agt cca gtt cat act tgc acc ac
TLR2 Arg753Gln Forward: cat tcc cca gcg ctt ctg caa gct c c 65
TLR2 Arg753Gln Reverse:: gga acc tag gac ttt atc gca gct c
TLR4 Asp299Gly Forward: agc ata ctt aga cta cta c ct cca tg 62
TLR4 Asp299Gly Reverse: aga aga ttt gag ttt caa tgt ggg
TLR4 Thr399Ile Forward: ggt tgc tgt tct caa agt gat ttt ggg a g a a 60
TLR4 Thr399Ile Reverse: gga aat cca gat gtt cta gtt gtt cta agc c