Figure 4

Electrophoretic separation of TLR2 and TLR4 gene PCR fragments after restriction endonuclease cleavage on agarose gel (a-d): (a) Agarose gel electrophoresis of the TLR2 Arg677Trp polymorphism (TLR2_C2180T): lane 1, restriction fragments in case of 1 wildtype individual; lane 3, 100 bp DNA ladder; lane 2, PCR product without (before) restriction. (b) Agarose gel electrophoresis of the TLR2 Arg753Gln polymorphism (TLR2_G2408A): lane 1, 100 bp DNA ladder; lanes 2 and 3, restriction fragments in case of two wildtype individuals; lane 4 individual heterozygous for the Arg753Gln mutation. (c) Agarose gel electrophoresis of the TLR4 Asp299Gly polymorphism (TLR4_A1287T): lane 1, 100 bp DNA ladder; lane 2 restriction fragments in case of an individual heterozygous for the Asp299Gly mutation; lane 3, restriction fragments in case of 1 wildtype individual. (d) Agarose gel electrophoresis of the TLR4 Thr399Ile polymorphism (TLR4_C13174T): lanes 1 and 3, restriction fragments in case of 2 wildtype individuals; lanes 2 and 4, restriction fragments in case of two individuals heterozygous for the Thr399Ile mutation; lane 5, 100 bp DNA ladder.