Table 4 List of primers used in this work
Primer name | Gene | Exon | F/R | Primer sequence (5’-3’) | Tm (°C) | Annealing Tm (°C) |
|---|---|---|---|---|---|---|
C1QA-02-F | C1qA | 02 | F | TTGTGTGCATGGGACTCAAG | 56 | 60 |
C1QA-02-R | C1qA | 02 | R | GGCCAAGTCAGGCCAAG | 58 | |
C1QA-03a-F | C1qA | 03a | F | TCCCTGAGGACCAGTAGGC | 60 | 60 |
C1QA-03a-R | C1qA | 03a | R | GGACAGGCAGATTTCCCAC | 58 | |
C1QA-03b-F | C1qA | 03b | F | TCATCTTCGACACGGTCATC | 56 | 60 |
C1QA-03b-R | C1qA | 03b | R | ATTTTACAGGCGGAGCATGG | 56 | |
C1QB-02-F | C1qB | 02 | F | GGATGCAGATGGAGGGATAG | 58 | 60 |
C1QB-02-R | C1qB | 02 | R | AGGCAACTGTGACTTGGGAG | 58 | |
C1QB-03a-F | C1qB | 03a | F | GCAGGCCTCCTTCTTTTGG | 58 | 60 |
C1QB-03a-R | C1qB | 03a | R | TCACGCACAGGTTCCCTC | 58 | |
C1QB-03b-F | C1qB | 03b | F | CAGACCATCCGCTTCGAC | 58 | 60 |
C1QB-03b-R | C1qB | 03b | R | GGGGTAGAGTGAGCGTTGC | 60 | |
C1QC-02-F | C1qC | 02 | F | ATCCATGGTGAGGCTCCTG | 58 | 60 |
C1QC-02-R | C1qC | 02 | R | CCCAGACAGACACTCTGATCC | 60 | |
C1QC-03a-F | C1qC | 03a | F | GTTCCCTGGAAGACACCCTC | 60 | 60 |
C1QC-03a-R | C1qC | 03a | R | TATGCGACGCGTGGTAGAC | 58 | |
C1QC-03b-F | C1qC | 03b | F | AGCCTGATCAGATTCAACGC | 56 | 60 |
C1QC-03b-R | C1qC | 03b | R | TGGCCAGTAAGGTGGGTCC | 60 |