Table 2 Spectrum analysis (MALDI-TOF & LC-MS) results 11 spots
S. No | Spot ID | Accession no (Database = NCBI nr) | Protein sequence name | Molecular mass (Dalton) | pI (Isoelectric point) | Sequence coverage MS | Intensity coverage | Score | No of peaks |
|---|---|---|---|---|---|---|---|---|---|
1. | A-10 (LC-MS) | gi|178775 | Pro-apo-lipoprotein [Homo sapiens] | 28944 | 5.13 | 7% | 213 | ||
2. | A-11 (MALDI-TOF) | gi|51095091 | Leiomodin 2(Cardiac) [Homo sapiens] | 59366 | 9.9 | 14.5% | 43.7% (25167 cnts) | 69.4 | 16 |
3. | A-13 (MALDI-TOF) | gi|78101270 | Chain B, Human Complement Component C3C [Homo sapiens] | 21596 | 5.8 | 18.1% | 36.3% (13319 cnts) | 68.0 | 17 |
4. | A-16 (LC-MS) | gi|269849769 | Epidermal cytokeratin 2 [Homo sapiens] | 59020 | 5.13 | 7% | - | 171 | - |
5. | A-17 (LC-MS) | gi|29836906 | Immunoglobulin kappa light chain variable region [Homo sapiens] | 8811 | 8.65 | 20% | - | 62 | - |
6. | A-18 (LC-MS) | gi|7331218 | Keratin 1[Homo sapiens] | 66149 | 8.16 | 3% | - | 63 | - |
7. | A-21 (MALDI-TOF) | gi|212374952 | Chain A, Crystal structure of Transthyretin Variant del 122[Homo sapiens] | 13798 | 5.35 | 48.8% | 33.4% (18627 cnts) | 85.4 | 15 |
8. | W (LC-MS) | gi|5542161 | Chain H, 1.9a Structure Of The Therapeutic Antibody Campath-1 h Fab In Complex With A Synthetic Peptide Antigen[Homo sapiens] | 23740 | 9.03 | 6% | - | 105 | |
9. | C7 (MALDI-TOF) | gi|222418558 | Bi-functional Methylene tetra hydrofolate de-hydrogenase/cyclohydrolase 2[Homo sapiens] | 37463 | 10.0 | 21.3% | 37.3% (18238 cnts) | 67.00 | 14 |
10 | D19 (MALDI-TOF) | gi|119589476 | Complement component C3, partial [Homo sapiens] | 144417 | 7.8 | 7.5% | 51.0% (34497 cnts) | 67.2 | 21 |
11. | D31 (LC-MS) | gi|18088326 | Retinol binding protein[Homo sapiens] | 23371 | 5.77 | 10% | - | 112 | - |